A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine

Autores
Medrano, E.E.; Burrone, O.R.; Ferrer, M.M.; Cafferata, E.G.A.; Algranati, I.D.
Año de publicación
1986
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of α-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain almost no ornithine decarboxylase (ODC) activity and concomitantly very low levels of putrescine and spermidine. Southern blot analysis and measurements of ODC activity and intracellular polyamine levels showed that the described mechanism of inducing resistance to DFMO triggered by CHM does not involve ODC gene amplification, altered transport of the drug or reduced affinity of the enzyme for DFMO. © 1986.
Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Burrone, O.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ferrer, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
FEBS Lett. 1986;206(1):106-110
Materia
Cycloheximide
Ornithine decarboxylase
Polyamine
α-Difluoromethylornithine resistance
cycloheximide
eflornithine
putrescine
spermidine
cell line
drug inhibition
drug resistance
fibroblast
in vitro study
nonhuman
priority journal
Animals
Cell Line
Cycloheximide
Drug Resistance
Eflornithine
Fibroblasts
Humans
Mice
Ornithine Decarboxylase
Putrescine
Spermidine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00145793_v206_n1_p106_Medrano
Acceder
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oai_identifier_str paperaa:paper_00145793_v206_n1_p106_Medrano
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidineMedrano, E.E.Burrone, O.R.Ferrer, M.M.Cafferata, E.G.A.Algranati, I.D.CycloheximideOrnithine decarboxylasePolyamineα-Difluoromethylornithine resistancecycloheximideeflornithineputrescinespermidinecell linedrug inhibitiondrug resistancefibroblastin vitro studynonhumanpriority journalAnimalsCell LineCycloheximideDrug ResistanceEflornithineFibroblastsHumansMiceOrnithine DecarboxylasePutrescineSpermidineTreatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of α-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain almost no ornithine decarboxylase (ODC) activity and concomitantly very low levels of putrescine and spermidine. Southern blot analysis and measurements of ODC activity and intracellular polyamine levels showed that the described mechanism of inducing resistance to DFMO triggered by CHM does not involve ODC gene amplification, altered transport of the drug or reduced affinity of the enzyme for DFMO. © 1986.Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Burrone, O.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ferrer, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1986info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00145793_v206_n1_p106_MedranoFEBS Lett. 1986;206(1):106-110reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_00145793_v206_n1_p106_MedranoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:55.775Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
title A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
spellingShingle A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
Medrano, E.E.
Cycloheximide
Ornithine decarboxylase
Polyamine
α-Difluoromethylornithine resistance
cycloheximide
eflornithine
putrescine
spermidine
cell line
drug inhibition
drug resistance
fibroblast
in vitro study
nonhuman
priority journal
Animals
Cell Line
Cycloheximide
Drug Resistance
Eflornithine
Fibroblasts
Humans
Mice
Ornithine Decarboxylase
Putrescine
Spermidine
title_short A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
title_full A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
title_fullStr A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
title_full_unstemmed A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
title_sort A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
dc.creator.none.fl_str_mv Medrano, E.E.
Burrone, O.R.
Ferrer, M.M.
Cafferata, E.G.A.
Algranati, I.D.
author Medrano, E.E.
author_facet Medrano, E.E.
Burrone, O.R.
Ferrer, M.M.
Cafferata, E.G.A.
Algranati, I.D.
author_role author
author2 Burrone, O.R.
Ferrer, M.M.
Cafferata, E.G.A.
Algranati, I.D.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cycloheximide
Ornithine decarboxylase
Polyamine
α-Difluoromethylornithine resistance
cycloheximide
eflornithine
putrescine
spermidine
cell line
drug inhibition
drug resistance
fibroblast
in vitro study
nonhuman
priority journal
Animals
Cell Line
Cycloheximide
Drug Resistance
Eflornithine
Fibroblasts
Humans
Mice
Ornithine Decarboxylase
Putrescine
Spermidine
topic Cycloheximide
Ornithine decarboxylase
Polyamine
α-Difluoromethylornithine resistance
cycloheximide
eflornithine
putrescine
spermidine
cell line
drug inhibition
drug resistance
fibroblast
in vitro study
nonhuman
priority journal
Animals
Cell Line
Cycloheximide
Drug Resistance
Eflornithine
Fibroblasts
Humans
Mice
Ornithine Decarboxylase
Putrescine
Spermidine
dc.description.none.fl_txt_mv Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of α-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain almost no ornithine decarboxylase (ODC) activity and concomitantly very low levels of putrescine and spermidine. Southern blot analysis and measurements of ODC activity and intracellular polyamine levels showed that the described mechanism of inducing resistance to DFMO triggered by CHM does not involve ODC gene amplification, altered transport of the drug or reduced affinity of the enzyme for DFMO. © 1986.
Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Burrone, O.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ferrer, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of α-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain almost no ornithine decarboxylase (ODC) activity and concomitantly very low levels of putrescine and spermidine. Southern blot analysis and measurements of ODC activity and intracellular polyamine levels showed that the described mechanism of inducing resistance to DFMO triggered by CHM does not involve ODC gene amplification, altered transport of the drug or reduced affinity of the enzyme for DFMO. © 1986.
publishDate 1986
dc.date.none.fl_str_mv 1986
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00145793_v206_n1_p106_Medrano
url http://hdl.handle.net/20.500.12110/paper_00145793_v206_n1_p106_Medrano
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv FEBS Lett. 1986;206(1):106-110
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618734610677760
score 13.070432

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