Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B

Autores
Maeto, C.A.; Knott, M.E.; Linero, F.N.; Ellenberg, P.C.; Scolaro, L.A.; Castilla, V.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.
Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Gen. Virol. 2011;92(9):2181-2190
Materia
cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00221317_v92_n9_p2181_Maeto
Acceder
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oai_identifier_str paperaa:paper_00221317_v92_n9_p2181_Maeto
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and BMaeto, C.A.Knott, M.E.Linero, F.N.Ellenberg, P.C.Scolaro, L.A.Castilla, V.cell proteinribonucleoproteinribonucleoprotein A1ribonucleoprotein A2ribonucleoprotein Bsmall interfering RNAunclassified drugvirus nucleoproteinArgentine hemorrhagic feverarticlecellular distributioncontrolled studygene overexpressionimmunoprecipitationJunin virusnonhumannucleocytoplasmic transportpersistent infectionpriority journalprotein expressionprotein protein interactionVero cellvirus cell interactionvirus replicationActive Transport, Cell NucleusAnimalsCercopithecus aethiopsHeterogeneous-Nuclear Ribonucleoprotein Group A-BHost-Pathogen InteractionsImmunoprecipitationJunin virusProtein BindingRNA, ViralVero CellsViral ProteinsVirus ReplicationArenavirusJunin virusRNA virusesHeterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_MaetoJ. Gen. Virol. 2011;92(9):2181-2190reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:58Zpaperaa:paper_00221317_v92_n9_p2181_MaetoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.222Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
spellingShingle Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
Maeto, C.A.
cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
title_short Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_fullStr Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full_unstemmed Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_sort Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
dc.creator.none.fl_str_mv Maeto, C.A.
Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
author Maeto, C.A.
author_facet Maeto, C.A.
Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
author_role author
author2 Knott, M.E.
Linero, F.N.
Ellenberg, P.C.
Scolaro, L.A.
Castilla, V.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
topic cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
dc.description.none.fl_txt_mv Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.
Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
url http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Gen. Virol. 2011;92(9):2181-2190
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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