Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias

Autores
De Geralnik, A.A.J.; Rossetti, M.V.; Del Carmen Batlle, A.M.
Año de publicación
1981
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981.
Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Int. J. Biochem. 1981;13(3):343-353
Materia
ammonia lyase
Ammonia Lyases
folic acid
glutathione
porphyrin
porphyrinogen
adult
article
biosynthesis
enzyme activation
Euglena gracilis
female
gel chromatography
heat
human
metabolism
molecular weight
porphyria
Adult
Ammonia-Lyases
Chromatography, Gel
Enzyme Activation
Euglena gracilis
Female
Folic Acid
Glutathione
Heat
Human
Molecular Weight
Porphyria
Porphyrinogens
Porphyrins
Support, Non-U.S. Gov't
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_0020711X_v13_n3_p343_DeGeralnik

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oai_identifier_str paperaa:paper_0020711X_v13_n3_p343_DeGeralnik
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyriasDe Geralnik, A.A.J.Rossetti, M.V.Del Carmen Batlle, A.M.ammonia lyaseAmmonia Lyasesfolic acidglutathioneporphyrinporphyrinogenadultarticlebiosynthesisenzyme activationEuglena gracilisfemalegel chromatographyheathumanmetabolismmolecular weightporphyriaAdultAmmonia-LyasesChromatography, GelEnzyme ActivationEuglena gracilisFemaleFolic AcidGlutathioneHeatHumanMolecular WeightPorphyriaPorphyrinogensPorphyrinsSupport, Non-U.S. Gov't1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981.Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1981info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnikInt. J. Biochem. 1981;13(3):343-353reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:55Zpaperaa:paper_0020711X_v13_n3_p343_DeGeralnikInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:56.236Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
title Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
spellingShingle Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
De Geralnik, A.A.J.
ammonia lyase
Ammonia Lyases
folic acid
glutathione
porphyrin
porphyrinogen
adult
article
biosynthesis
enzyme activation
Euglena gracilis
female
gel chromatography
heat
human
metabolism
molecular weight
porphyria
Adult
Ammonia-Lyases
Chromatography, Gel
Enzyme Activation
Euglena gracilis
Female
Folic Acid
Glutathione
Heat
Human
Molecular Weight
Porphyria
Porphyrinogens
Porphyrins
Support, Non-U.S. Gov't
title_short Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
title_full Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
title_fullStr Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
title_full_unstemmed Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
title_sort Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
dc.creator.none.fl_str_mv De Geralnik, A.A.J.
Rossetti, M.V.
Del Carmen Batlle, A.M.
author De Geralnik, A.A.J.
author_facet De Geralnik, A.A.J.
Rossetti, M.V.
Del Carmen Batlle, A.M.
author_role author
author2 Rossetti, M.V.
Del Carmen Batlle, A.M.
author2_role author
author
dc.subject.none.fl_str_mv ammonia lyase
Ammonia Lyases
folic acid
glutathione
porphyrin
porphyrinogen
adult
article
biosynthesis
enzyme activation
Euglena gracilis
female
gel chromatography
heat
human
metabolism
molecular weight
porphyria
Adult
Ammonia-Lyases
Chromatography, Gel
Enzyme Activation
Euglena gracilis
Female
Folic Acid
Glutathione
Heat
Human
Molecular Weight
Porphyria
Porphyrinogens
Porphyrins
Support, Non-U.S. Gov't
topic ammonia lyase
Ammonia Lyases
folic acid
glutathione
porphyrin
porphyrinogen
adult
article
biosynthesis
enzyme activation
Euglena gracilis
female
gel chromatography
heat
human
metabolism
molecular weight
porphyria
Adult
Ammonia-Lyases
Chromatography, Gel
Enzyme Activation
Euglena gracilis
Female
Folic Acid
Glutathione
Heat
Human
Molecular Weight
Porphyria
Porphyrinogens
Porphyrins
Support, Non-U.S. Gov't
dc.description.none.fl_txt_mv 1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981.
Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description 1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981.
publishDate 1981
dc.date.none.fl_str_mv 1981
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnik
url http://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnik
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Int. J. Biochem. 1981;13(3):343-353
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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