A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing

• Autores: Landau, A.M.; Lokstein, H.; Scheller, H.V.; Lainez, V.; Maldonado, S.; Prina, A.R.
• Año de publicación: 2009
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plant Physiol. 2009;151(4):1802-1811
• Materia: messenger RNA; pigment; plant extract; vegetable protein; article; barley; chloroplast; cytoplasm; gene expression regulation; genetics; germination; growth, development and aging; immunoblotting; inheritance; metabolism; mutation; nucleotide sequence; phenotype; photochemistry; photosynthesis; photosystem I; plant gene; RNA splicing; seedling; spectrofluorometry; temperature; thylakoid; ultrastructure; Base Sequence; Chloroplasts; Cytoplasm; Gene Expression Regulation, Plant; Genes, Plant; Germination; Hordeum; Immunoblotting; Inheritance Patterns; Mutation; Phenotype; Photochemistry; Photosynthesis; Photosystem I Protein Complex; Pigments, Biological; Plant Extracts; Plant Proteins; RNA Splicing; RNA, Messenger; Seedling; Spectrometry, Fluorescence; Temperature; Thylakoids; Hordeum; Hordeum vulgare
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

A cytoplasmically inherited chlorophyll-deficient mutant of barley (Hordeum vulgare) termed cytoplasmic line 3 (CL3), displaying a viridis (homogeneously light-green colored) phenotype, has been previously shown to be affected by elevated temperatures. In this article, biochemical, biophysical, and molecular approaches were used to study the CL3 mutant under different temperature and light conditions. The results lead to the conclusion that an impaired assembly of photosystem I (PSI) under higher temperatures and certain light conditions is the primary cause of the CL3 phenotype. Compromised splicing of ycf3 transcripts, particularly at elevated temperature, resulting from a mutation in a noncoding region (intron 1) in the mutant ycf3 gene results in a defective synthesis of Ycf3, which is a chaperone involved in PSI assembly. The defective PSI assembly causes severe photoinhibition and degradation of PSII. © 2009 American Society of Plant Biologists.
Fil:Landau, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Lainez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.