Xanthan induces plant susceptibility by suppressing callose deposition

• Autores: Yun, M.H.; Torres, P.S.; El Oirdi, M.; Rigano, L.A.; Gonzalez-Lamothe, R.; Marano, M.R.; Castagnaro, A.P.; Dankert, M.A.; Bouarab, K.; Vojnov, A.A.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plant Physiol. 2006;141(1):178-187
• Materia: Diseases; Enzyme inhibition; Plants (botany); Arabidopsis plants; Callose deposition; Xanthomonas pathogenesis; Polysaccharides; bacterial polysaccharide; callose; deoxyglucose; glucan; xanthan; amino acid sequence; Arabidopsis; article; chemistry; drug effect; gene deletion; histology; metabolism; microbiology; necrosis; pathogenicity; physiology; plant leaf; tobacco; Xanthomonas campestris; Amino Acid Sequence; Arabidopsis; Deoxyglucose; Glucans; Necrosis; Plant Leaves; Polysaccharides, Bacterial; Sequence Deletion; Tobacco; Xanthomonas campestris; Callose; Diseases; Enzymatic Activity; Xanthan Gum; Arabidopsis; Arabidopsis thaliana; Bacteria (microorganisms); Nicotiana benthamiana; Xanthomonas; Xanthomonas campestris
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Xanthan is the major exopolysaccharide secreted by Xanthomonas spp. Despite its diverse roles in bacterial pathogenesis of plants, little is known about the real implication of this molecule in Xanthomonas pathogenesis. In this study we show that in contrast to Xanthomonas campestris pv campestris strain 8004 (wild type), the xanthan minus mutant (strain 8397) and the mutant strain 8396, which is producing truncated xanthan, fail to cause disease in both Nicotiana benthamiana and Arabidopsis (Arabidopsis thaliana) plants. In contrast to wild type, 8397 and 8396 strains induce callose deposition in N. benthamiana and Arabidopsis plants. Interestingly, treatment with xanthan but not truncated xanthan, suppresses the accumulation of callose and enhances the susceptibility of both N. benthamiana and Arabidopsis plants to 8397 and 8396 mutant strains. Finally, in concordance, we also show that treatment with an inhibitor of callose deposition previous to infection induces susceptibility to 8397 and 8396 strains. Thus, xanthan suppression effect on callose deposition seems to be important for Xanthomonas infectivity. © 2006 American Society of Plant Biologists.
Fil:Torres, P.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Rigano, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vojnov, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.