Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants

• Autores: Ruiz, J.A.; Fernández, R.O.; Nikel, P.I.; Méndez, B.S.; Pettinari, M.J.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: FEMS Microbiol. Lett. 2006;258(1):55-60
• Materia: arcA; O2 consumption; PHB; ROS; Toluidine blue; poly(3 hydroxybutyric acid); tolonium chloride; aerobic metabolism; article; controlled study; Escherichia coli; gene mutation; genetic code; molecular dynamics; nonhuman; oxidation reduction reaction; oxygen consumption; phenotype; polymerization; priority journal; Bacterial Outer Membrane Proteins; Escherichia coli; Escherichia coli Proteins; Hydroxybutyrates; Mutation; Oxygen Consumption; Phenotype; Polyesters; Reactive Oxygen Species; Recombination, Genetic; Repressor Proteins; Arca; Escherichia coli
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.