Authors: Fernandez, María Elena; Liron, Juan Pedro; Prando, Alberto; Rogberg Muñoz, Andres; Peral Garcia, Pilar; Baldo A; Giovambattista, Andres
Publication Date: 2014.
Language: English.
Abstract:
In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that exert their effect on the hypothalamic–pituitary–gonadal (HPG) axis, and several aspects of regulation of the HPG axis are yet to be fully elucidated. The objective of this work was to identify new genes associated with age at puberty. Angus males (N=276) were weighted and scrotal circumference measured every month. When first bull calves reached 26 cm of scrotal circumference, sperm quality was added to the monthly measurements for the next three months. Based on sperm quality, two groups corresponding to the top and bottom 6.5% of the phenotypic distribution curve were selected to perform a genome-wide association study using a selective DNA approach, to be used as an exploratory analysis. Based on the results of this preliminary study, five SNPs located within BTA3, 20 and 24 associated regions were genotyped in the 276 bulls using pyrosequencing methods. This analysis allowed us to confirm the association of a BTA20 region with age at puberty estimated at C 50 million. The associated SNP explained 1.69% of the genetic variance of age at puberty at C 50 million. The associated region contained within and nearby positional candidate genes (ISL1, PELO, FST, SPZ1) that may be involved in spermatogenesis. Our results should encourage further investigation to confirm the role of these genes in order to increase our knowledge on genetic control of bovine puberty.
Author affiliation: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Prando, Alberto. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Baldo A. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Ridruejo, Ezequiel; Solano, Angela Rosario; Marciano, Sebastián; Galdame, Omar; Adrover, Raúl; Cocozzella, Daniel; Delettieres, Dreanina; Martínez, Alfredo; Gadano, Adrián; Mandó, Oscar G.; Silva, Marcelo O.
Publication Date: 2011.
Language: English.
Abstract:
BACKGROUND AND AIMS: Genetic variations in the interleukin 28B (IL28B) gene have been associated with viral response to PEG-interferon-α/ribavirin (PR) therapy in hepatitis C virus (HCV) genotype 1 infected patients from North America, Europe and Asia. The importance of these IL28B variants for Argentine patients remains unknown. MATERIAL AND METHODS: IL28B host genotypes (rs8099917 and rs12979860) were determined in a population of Argentine patients with European ancestry. Results were analyzed looking for their association with sustained virologic response (SVR) to PR therapy and compared with other baseline hosts' biochemical, histological and virological predictors of response. RESULTS: We studied 102 patients, 60% were men, and 40% of them were rs8099917 TT and 18% rs12979860 CC. Mean baseline serum HCV RNA was 1.673.092 IU/mL and mean F score was: 2.10 ± 1.18 (21% cirrhotic). SVR rate was higher in rs8099917 TT genotypes (55%) when compared to GT/GG (25%) (p = 0.002) and in rs1512979860 CC (64%) than in CT/TT (30%) (p = 0.004). The univariate analysis showed that rs8099917 TT (OR 3.7; 95 %CI 1.5-8.7; p = 0.002), rs12979860 CC (OR 4.6; 95%CI 1.5-13.7; p = 0.006), low viral load (OR 4.6; 95% CI 1.7-12.6; p = 0.002) and F0-2 (OR 8.5; 95% CI 2.3-30.6; p = 0.001) were significantly associated with SVR. In the multivariate analysis, rs12979860 CC, rs8099917 TT, viral load < 400.000 IU/mL and F0-2 were associated with SVR rates (p = 0.029, p = 0.012, p = 0.013 and p = 0.004, respectively). CONCLUSION: IL28B host genotypes should be added to baseline predictors of response to PR therapy in Latin American patients with European ancestry.
Author affiliation: Ridruejo, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Hospital Universitario Austral; Argentina
Author affiliation: Solano, Angela Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET.; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Author affiliation: Marciano, Sebastián. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina. Hospital Italiano; Argentina
Author affiliation: Galdame, Omar. Hospital Italiano; Argentina
Author affiliation: Adrover, Raúl. Centro de Hepatologia; Argentina
Author affiliation: Cocozzella, Daniel. Centro de Hepatologia; Argentina
Author affiliation: Delettieres, Dreanina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Author affiliation: Martínez, Alfredo. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Author affiliation: Gadano, Adrián. Hospital Italiano; Argentina
Author affiliation: Mandó, Oscar G.. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Author affiliation: Silva, Marcelo O.. Hospital Universitario Austral; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Papaleo Mazzucco, J.; Goszczynski, Daniel Estanislao; Ripoli, María Verónica; Melucci, L. M.; Pardo, A. M.; Colatto, E.; Rogberg Muñoz, Andres; Mezzadra, C. A.; Depetris, G. J.; Giovambattista, Guillermo; Villarreal, E. L.
Publication Date: 2016.
Language: English.
Abstract:
Grazing steers from Angus and Hereford breeds, their cross-breeds and a three-way cross-breed (Limousin × Angus–Hereford) were measured for growth, carcass and meat quality traits. Breed effects were studied, and the association of SNPs with fat deposition and fatty acid (FA) composition (leptin, melanocortin-4 receptor, stearoyl-CoA desaturase, FA synthase and thyroglobulin) was tested. Limousin cross-breed showed the greatest final body weight, ultrasound rib eye area, dressing percentage, carcass and leg length, and the lowest backfat thickness and intramuscular fat content. Genetic groups had similar pH, shear force, cooking loss, L* and b* and n-6:n-3 ratio. Meat from 1/2-Angus presented greater a* than Limousin cross-breed. Whereas Angus had the highest total SFA content, Hereford had the lowest total SFA and the highest total MUFA. Limousin cross-breed had greater content of several individual PUFAs, total PUFA, n-6 and n-3 FA than Angus and 1/2-Angus. Leptin and FA synthase were associated with some FAs, supporting their influence over fat metabolism for grazing animals.
Author affiliation: Papaleo Mazzucco, J.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Author affiliation: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Melucci, L. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Produccion Animal; Argentina
Author affiliation: Pardo, A. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Author affiliation: Colatto, E.. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Produccion Animal; Argentina
Author affiliation: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina
Author affiliation: Mezzadra, C. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Author affiliation: Depetris, G. J.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Author affiliation: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Villarreal, E. L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires. Estación Experimental Agropecuaria Balcarce; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Vera Lozada, Gabriela; Minnicelli, Carolina; Segges, Priscilla; Stefanoff, Gustavo; Kristcevic, Flavia; Ezpeleta, Joaquin; Tapia, Elizabeth; Niedobitek, Gerald; Barros, Mario Henrique; Hassan, Rocio
Publication Date: 2018.
Language: English.
Abstract:
Interleukin-10 (IL10) is an immune regulatory cytokine. Single nucleotide polymorphisms (SNPs) in IL10 promoter have been associated with prognosis in adult classical Hodgkin lymphoma (cHL). We analyzed IL10 SNPs −1082 and −592 in respect of therapy response, gene expression and tumor microenvironment (TME) composition in 98 pediatric patients with cHL. As confirmatory results, we found that −1082AA/AG; −592CC genotypes and ATA haplotype were associated with unfavourable prognosis: Progression-free survival (PFS) was shorter in −1082AA+AG (72.2%) than in GG patients (100%) (P = 0.024), and in −592AA (50%) and AC (74.2%) vs. CC patients (87.0%) (P = 0.009). In multivariate analysis, the −592CC genotype and the ATA haplotype retained prognostic impact (HR: 0.41, 95% CI 0.2–0.86; P = 0.018, and HR: 3.06 95% CI 1.03–9.12; P = 0.044, respectively). Our analysis further led to some new observations, namely: (1) Low IL10 mRNA expression was associated with −1082GG genotype (P = 0.014); (2) IL10 promoter polymorphisms influence TME composition;−1082GG/−592CC carriers showed low numbers of infiltrating cells expressing MAF transcription factor (20 vs. 78 and 49 vs. 108 cells/mm2, respectively; P< 0.05); while ATA haplotype (high expression) associated with high numbers of MAF+ cells (P = 0.005). Specifically, −1082GG patients exhibited low percentages of CD68+MAF+ (M2-like) intratumoral macrophages (15.04% vs. 47.26%, P = 0.017). Considering ours as an independent validation cohort, our results give support to the clinical importance of IL10 polymorphisms in the full spectrum of cHL, and advance the concept of genetic control of microenvironment composition as a basis for susceptibility and therapeutic response.
Author affiliation: Vera Lozada, Gabriela. Instituto Nacional de Cancer; Brasil
Author affiliation: Minnicelli, Carolina. Instituto Nacional de Cancer; Brasil. Universidade Federal do Rio Grande do Norte; Brasil
Author affiliation: Segges, Priscilla. Instituto Nacional de Cancer; Brasil
Author affiliation: Stefanoff, Gustavo. Instituto Nacional de Cancer; Brasil
Author affiliation: Kristcevic, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Author affiliation: Ezpeleta, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Author affiliation: Tapia, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Author affiliation: Niedobitek, Gerald. Unfallkrankenhaus Berlin; Alemania
Author affiliation: Barros, Mario Henrique. Unfallkrankenhaus Berlin; Alemania
Author affiliation: Hassan, Rocio. Instituto Nacional de Cancer; Brasil
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Grattapaglia, Dario; Silva Junior, Orzenil B.; Resende, Rafael T.; Cappa, Eduardo Pablo; Müller, Bárbara S. F.; Tan, Biyue; Isik, Fikret; Ratcliffe, Blaise; El-Kassaby, Yousry A.
Publication Date: 2018.
Language: English.
Abstract:
Forest tree breeding has been successful at delivering genetically improved material for multiple traits based on recurrent cycles of selection, mating, and testing. However, long breeding cycles, late flowering, variable juvenile-mature correlations, emerging pests and diseases, climate, and market changes, all pose formidable challenges. Genetic dissection approaches such as quantitative trait mapping and association genetics have been fruitless to effectively drive operational marker-assisted selection (MAS) in forest trees, largely because of the complex multifactorial inheritance of most, if not all traits of interest. The convergence of high-throughput genomics and quantitative genetics has established two new paradigms that are changing contemporary tree breeding dogmas. Genomic selection (GS) uses large number of genome-wide markers to predict complex phenotypes. It has the potential to accelerate breeding cycles, increase selection intensity and improve the accuracy of breeding values. Realized genomic relationships matrices, on the other hand, provide innovations in genetic parameters’ estimation and breeding approaches by tracking the variation arising from random Mendelian segregation in pedigrees. In light of a recent flow of promising experimental results, here we briefly review the main concepts, analytical tools and remaining challenges that currently underlie the application of genomics data to tree breeding. With easy and cost-effective genotyping, we are now at the brink of extensive adoption of GS in tree breeding. Areas for future GS research include optimizing strategies for updating prediction models, adding validated functional genomics data to improve prediction accuracy, and integrating genomic and multi-environment data for forecasting the performance of genetic material in untested sites or under changing climate scenarios. The buildup of phenotypic and genome-wide data across large-scale breeding populations and advances in computational prediction of discrete genomic features should also provide opportunities to enhance the application of genomics to tree breeding.
Author affiliation: Grattapaglia, Dario. EMBRAPA Recursos Genéticos e Biotecnologia; Brasil. Universidade Católica de Brasília. Programa de Ciências Genômicas e Biotecnologia; Brasil. Universidade de Brasília. Departamento de Biologia Celular; Brasil. North Carolina State University. Department of Forestry and Environmental Resources; Estados Unidos
Author affiliation: Silva-Junior, Orzenil B. EMBRAPA Recursos Genéticos e Biotecnologia; Brasil. Universidade Católica de Brasília. Programa de Ciências Genômicas e Biotecnologia; Brasil
Author affiliation: Resende, Rafael T. EMBRAPA Recursos Genéticos e Biotecnologia; Brasil
Author affiliation: Cappa, Eduardo Pablo. INTA. Instituto de Recursos Biológicos; Argentina
Author affiliation: Müller, Bárbara S. F. EMBRAPA Recursos Genéticos e Biotecnologia; Brasil. Universidade de Brasília. Departamento de Biologia Celular; Brasil
Author affiliation: Tan, Biyue. Stora Enso AB. Biomaterials Division; Suecia
Author affiliation: Isik, Fikret. North Carolina State University. Department of Forestry and Environmental Resources; Estados Unidos
Author affiliation: Rateliffe, Blaise. University of British Columbia. Faculty of Forestry. Department of Forest and Conservation Sciences; Canadá
Author affiliation: El-Kassaby, Yousry A. University of British Columbia. Faculty of Forestry. Department of Forest and Conservation Sciences; Canadá
Keywords: Quantitative Genetics; Quantitative Trait Loci; Forest Trees; Genética Cuantitativa; Loci de Rasgos Cuantitativos; Árboles Forestales; Genomic Selection; Tree Breeding; Whole-genome Regression; Single Nucleotide Polymorphisms; Marker Assisted Selection; Realized Genomic Relationship; Selección Genómica; Cría de Arboles; Regresión de Todo el Genoma; Polimorfismos de un Sólo Nucleótido; Selección Asistida por Marcador; Relación Genómica Realizada.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Raschia, Maria Agustina; Nani, Juan Pablo; Maizon, Daniel Omar; Beribe, Maria Jose; Amadio, Ariel; Poli, Mario Andres
Publication Date: 2018.
Language: English.
Abstract:
Research on loci influencing milk production traits of dairy cattle is one of the main topics of investigation in livestock. Many genomic regions and polymorphisms associated with dairy production have been reported worldwide. In this context, the purpose of this study was to identify candidate loci associated with milk yield in Argentinean dairy cattle. A database of candidate genes and single nucleotide polymorphisms (SNPs) for milk production and composition was developed. Thirty-nine SNPs belonging to 22 candidate genes were genotyped on 1643 animals (Holstein and Holstein x Jersey). The genotypes obtained were subjected to association studies considering the whole population and discriminating the population by Holstein breed percentage. Phenotypic data consisted of milk production values recorded during the first lactation of 1156 Holstein and 462 Holstein x Jersey cows from 18 dairy farms located in the central dairy area of Argentina. From these records, 305-day cumulative milk production values were predicted.
EEA Pergamino
Author affiliation: Raschia, Maria Agustina. INTA. Instituto de Genética; Argentina.
Author affiliation: Nani, Juan Pablo. INTA. Estación Experimental Agropecuaria Rafaela; Argentina.
Author affiliation: Maizon, Daniel Omar INTA. Estación Experimental Agropecuaria Anguil; Argentina.
Author affiliation: Beribe, Maria Jose. INTA. Estación Experimental Agropecuaria Pergamino. Departamento de Estadística; Argentina
Author affiliation: Amadio, Ariel. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Author affiliation: Poli, Mario Andres. INTA. Instituto de Genética; Argentina.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Paladino, Natalia; Flores, Ana Claudia; Fainboim, Hugo; Schroder, Teresa; Cuarterolo, Miriam; Lezama, Carol; Gonzáles Ballerga, Esteban; Levi, Diana; Tanno, Hugo; Costanzo, Gabriel Gustavo; Arruvito, Maria Lourdes; Fainboim, Leonardo
Publication Date: 2010.
Language: English.
Abstract:
We previously reported that paediatric (PAH) and adult (AAH) forms of type I autoimmune hepatitis (AH) have different HLA-associations and clinical outcome. In the present study we investigated the role of TGF-β1 genetic polymorphisms in the different outcome of PAH and AAH. We found a significant increase of "high producer" 25GG genotype in PAH and 10CC in AAH. Low inflammation and low fibrosis in AAH was associated with the increase of codon 10CC (high producer) and codon 25CC (low producer) genotypes. The analysis in AAH of the two positions-haplotypes revealed that combined presence of 25GG and 10CC seems to neutralize the 10CC effect which remained in AAH having the 10CC+-25GG- haplotype. Altogether these results may explain, at least partially, the different clinical outcome of AAH and PAH.
Author affiliation: Paladino, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Author affiliation: Flores, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Author affiliation: Fainboim, Hugo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Author affiliation: Schroder, Teresa. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Author affiliation: Cuarterolo, Miriam. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Author affiliation: Lezama, Carol. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Author affiliation: Gonzáles Ballerga, Esteban. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Author affiliation: Levi, Diana. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
Author affiliation: Tanno, Hugo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Author affiliation: Costanzo, Gabriel Gustavo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Author affiliation: Arruvito, Maria Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Author affiliation: Fainboim, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas