Publication Date: 2017.
In this paper, the utility of a partial sequence of the COI gene, the DNA barcoding region, for the identification of species of black flies in the austral region was assessed. Twenty-eight morphospecies were analyzed: eight of the genus Austrosimulium (four species in the subgenus Austrosimulium s. str., three species in the subgenus Novaustrosimulium, and one species unassigned to subgenus), two of the genus Cnesia, eight of Gigantodax, three of Paracnephia, one of Paraustrosimulium, and six of Simulium (subgenera Morops, Nevermannia, and Pternaspatha). The neighbour-joining tree derived from the DNA barcode sequences grouped most specimens according to species or species groups recognized by morphotaxonomic studies. Intraspecific sequence divergences within morphologically distinct species ranged from 0% to 1.8%, while higher divergences (2%-4.2%) in certain species suggested the presence of cryptic diversity. The existence of well-defined groups within S. simile revealed the likely inclusion of cryptic diversity. DNA barcodes also showed that specimens identified as C. dissimilis, C. nr. pussilla, and C. ornata might be conspecific, suggesting possible synonymy. DNA barcoding combined with a sound morphotaxonomic framework would provide an effective approach for the identification of black flies in the region.
Author affiliation: Hernández Triana, Luis M.. Animal and Plant Health Agency; Reino Unido
Author affiliation: Montes de Oca, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administración de Parques Nacionales. Parque Nacional "Nahuel Huapi"; Argentina
Author affiliation: Prosser, Sean W. J.. University of Guelph; Canadá
Author affiliation: Hebert, Paul David Neil. University of Guelph; Canadá
Author affiliation: Gregory, T. Ryan. University of Guelph; Canadá
Author affiliation: McMurtrie, Shelley. EOS Ecology; Nueva Zelanda
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas