Authors: Gómez Raccio, Andrea C.; Orellana, Julio Cesar; Liberatore, Diana; Bezrodnik, Liliana; Marciano, Beatriz E.; Huang, Chiung Yu; Joshi, Gyan; Rezaei, Nima; Costa Carvalho, Beatriz; Cunha, Luciana; Pinto, Jorge A.; Espinosa Padilla, Sara E.; Hernandez Nieto, Leticia; Elfeky, Reem A.; Ariga, Tadashi; Toshio, Heike; Dogu, Figen; Cipe, Funda; Formankova, Renata; Nuñez Nuñez, M. Enriqueta; Gonçalo Marques, Jose; Pereira, María I.; Listello, Viviana; Slatter, Mary A.; Nademi, Zohreh; Kowalczyk, Danuta; Fleisher, Thomas A.; Davies, Graham; Neven, Bénédicte; Rosenzweig, Sergio D.
Publication Date: 2014.
BACKGROUND: Severe combined immunodeficiency (SCID) is a syndrome characterized by profound T-cell deficiency. BCG vaccine is contraindicated in patients with SCID. Because most countries encourage BCG vaccination at birth, a high percentage of patients with SCID are vaccinated before their immune defect is detected. OBJECTIVES: We sought to describe the complications and risks associated with BCG vaccination in patients with SCID. METHODS: An extensive standardized questionnaire evaluating complications, therapeutics, and outcomes regarding BCG vaccination in patients given a diagnosis of SCID was widely distributed. Summary statistics and association analysis was performed. RESULTS: Data on 349 BCG-vaccinated patients with SCID from 28 centers in 17 countries were analyzed. Fifty-one percent of the patients had BCG-associated complications, 34% disseminated and 17% localized (a 33,000- and 400-fold increase, respectively, over the general population). Patients receiving early vaccination (≤1 month) showed an increased prevalence of complications (P = .006) and death caused by BCG-associated complications (P < .0001). The odds of experiencing complications among patients with T-cell numbers of 250/μL or less at diagnosis was 2.1 times higher (95% CI, 1.4-3.4 times higher; P = .001) than among those with T-cell numbers of greater than 250/μL. BCG-associated complications were reported in 2 of 78 patients who received antimycobacterial therapy while asymptomatic, and no deaths caused by BCG-associated complications occurred in this group. In contrast, 46 BCG-associated deaths were reported among 160 patients treated with antimycobacterial therapy for a symptomatic BCG infection (P < .0001). CONCLUSIONS: BCG vaccine has a very high rate of complications in patients with SCID, which increase morbidity and mortality rates. Until safer and more efficient antituberculosis vaccines become available, delay in BCG vaccination should be considered to protect highly vulnerable populations from preventable complications.
Author affiliation: Gómez Raccio, Andrea C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños ; Argentina
Author affiliation: Orellana, Julio Cesar. Provincia de Córdoba. Hospital de Niños de la Santísima Trinidad. División de Alergia e Inmunología Clínica; Argentina
Author affiliation: Liberatore, Diana. Hospital Italiano; Argentina
Author affiliation: Bezrodnik, Liliana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Marciano, Beatriz E.. National Institutes of Health; Estados Unidos
Author affiliation: Huang, Chiung Yu. National Institutes of Health; Estados Unidos
Author affiliation: Joshi, Gyan. National Institutes of Health; Estados Unidos
Author affiliation: Rezaei, Nima. Teheran University of Medical Sciences. Children's Medical Center Hospital. Pediatric Center of Excellence; Irán
Author affiliation: Costa Carvalho, Beatriz. Federal University of São Paulo; Brasil
Author affiliation: Cunha, Luciana. Federal University of Minas Gerais; Brasil
Author affiliation: Pinto, Jorge A.. Federal University of Minas Gerais; Brasil
Author affiliation: Espinosa Padilla, Sara E.. Secretaría de Salud. Instituto Nacional de Pediatría; México
Author affiliation: Hernandez Nieto, Leticia. Secretaría de Salud. Instituto Nacional de Pediatría; México
Author affiliation: Elfeky, Reem A.. Ain Shams University; Egipto
Author affiliation: Ariga, Tadashi. Hokkaido University Graduate School of Medicine; Japón
Author affiliation: Toshio, Heike. Kyoto University Hospital; Japón
Author affiliation: Dogu, Figen. Ankara University Medical School; Turquía
Author affiliation: Cipe, Funda. Ankara University Medical School; Turquía
Author affiliation: Formankova, Renata. Charles University; República Checa. University Hospital Motol; República Checa
Author affiliation: Nuñez Nuñez, M. Enriqueta. Western National Medical Center; México
Author affiliation: Gonçalo Marques, Jose. Santa María Hospital. Lisbon Academic Center; Portugal
Author affiliation: Pereira, María I.. Provincia de Córdoba. Hospital de Niños de la Santísima Trinidad. División de Alergia e Inmunología Clínica; Argentina
Author affiliation: Listello, Viviana. Provincia de Córdoba. Hospital de Niños de la Santísima Trinidad. División de Alergia e Inmunología Clínica; Argentina
Author affiliation: Slatter, Mary A.. Great North Children's Hospital; Reino Unido
Author affiliation: Nademi, Zohreh. Great North Children's Hospital; Reino Unido
Author affiliation: Kowalczyk, Danuta. Children's University Hospital. Department of Clinical Immunology and Transplantology; Polonia
Author affiliation: Fleisher, Thomas A.. National Institutes of Health; Estados Unidos
Author affiliation: Davies, Graham. Great Ormond Street Hospital for Children; Reino Unido
Author affiliation: Neven, Bénédicte. Necker Hospital. Immunology-Hematology and Rheumatology Service; Francia
Author affiliation: Rosenzweig, Sergio D.. National Institute of Health. National Institute of Allergy and Infectious. Laboratory of Host Defenses. Primary Immunodeficiency Clinic and Infectious Diseases Susceptibility Unit; Estados Unidos
Keywords: Hematopoietic stem cell transplant; IL-2 receptor g; Recombination-activating gene; Severe combined immunodeficiency; Primary immunodeficiency; Vaccine; BCG; Immune reconstitution syndrome; Mycobacteria; Newborn screening; Medicina Critica y de Emergencia; Medicina Clínica; CIENCIAS MÉDICAS Y DE LA SALUD.
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Publication Date: 2015.
The effect of perforation, drying temperature, and rehydration temperature on the rehydration kinetics of Rosa rubiginosa fruits was investigated. Before drying, half of the fruit sample was perforated three times at equidistant points along the equatorial plane of the fruit, in order to speed up the drying process. Samples were dried at various air temperatures (60, 70, and 80°C), with an air velocity of 5 m/s and 5% relative humidity. Then, dried samples were rehydrated at different temperatures (20, 40, 60, and 80°C). The rehydration kinetics was fitted by two empirical models, Peleg and Weibull, and both represented the phenomenon well, in perforated and nonperforated fruits. Regardless of the drying temperature, the higher the rehydration temperature of rose hip fruits, perforated or not, the higher the water absorption capacity. Temperature dependence of the kinetic parameters was Ea = 47.5 kJ/mol (Peleg) and 55.9 kJ/mol (Weibull) for nonperforated fruits and Ea = 40.1 kJ/mol (Peleg) and 45.5 kJ/mol (Weibull) for perforated fruits; thus, perforated fruits were influenced more by rehydration temperature than nonperforated fruits. Perforated fruits rehydrated 30% faster than nonperforated fruits.
Author affiliation: Ohaco Dominguez, Elizabeth Haydee. Universidad Nacional del Comahue. Facultad de Tecnología de Alimentos; Argentina.
Author affiliation: Ichiyama, Beatriz. Universidad Nacional del Comahue. Facultad de Tecnologia de los Alimentos; Argentina
Author affiliation: Lozano, Jorge Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina. Universidad Nacional del Sur; Argentina
Author affiliation: De Michelis, Antonio. INTA. Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural El Bolsón; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Publication Date: 2016.
Thermosensors detect temperature changes and trigger cellular responses crucial for survival at different temperatures. The thermosensor DesK is a transmembrane (TM) histidine kinase which detects a decrease in temperature through its TM segments (TMS). Here, we address a key issue: how a physical stimulus such as temperature can be converted into a cellular response. We show that the thickness of Bacillus lipid membranes varies with temperature and that such variations can be detected by DesK with great precision. On the basis of genetic studies and measurements of in vitro activity of a DesK construct with a single TMS (minimal sensor DesK [MS-DesK]), reconstituted in liposomes, we propose an interplay mechanism directed by a conserved dyad, phenylalanine 8-lysine 10. This dyad is critical to anchor the only transmembrane segment of the MS-DesK construct to the extracellular water-lipid interphase and is required for the transmembrane segment of MS-DesK to function as a caliper for precise measurement of membrane thickness. The data suggest that positively charged lysine 10, which is located in the hydrophobic core of the membrane but is close to the water-lipid interface, pulls the transmembrane region toward the water phase to localize its charge at the interface. Nevertheless, the hydrophobic residue phenylalanine 8, located at the N-terminal extreme of the TMS, has a strong tendency to remain in the lipid phase, impairing access of lysine 10 to the water phase. The outcome of this interplay is a fine-tuned sensitivity to membrane thickness that elicits conformational changes that favor different signaling states of the protein.
Author affiliation: Inda, María Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina
Author affiliation: Oliveira, Rafael Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Author affiliation: de Mendoza, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Author affiliation: Cybulski, Larisa Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas