"Pollution microbiology" » "Pollution microbiologia"
Authors: Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; Åström, Johan; Blanch, Anicet R.; Blöschl, Günter; Byamukama, Denis; Coakley, Tricia; Ferguson, Christobel; Goshu, Goraw; Ko, GwangPyo; de Roda Husman, Ana Maria; Mushi, Douglas; Poma, Hugo Ramiro; Pradhan, Bandana; Rajal, Verónica Beatriz; Schade, Margit A.; Sommer, Regina; Taylor, Huw; Toth, Erika M.; Vrajmasu, Virgil; Wuertz, Stefan; Mach, Robert L.; Farnleitner, Andreas H.
Publication Date: 2013.
Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.
Author affiliation: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
Author affiliation: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
Author affiliation: Bauer, Johanna M.. Vienna University of Technology; Austria
Author affiliation: Schuster, Nathalie. Vienna University of Technology; Austria
Author affiliation: Ahmed, Warish. CSIRO Land and Water; Australia
Author affiliation: Åström, Johan. Chalmers University Of Technology; Suecia
Author affiliation: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; España
Author affiliation: Blöschl, Günter. Vienna University of Technology; Austria
Author affiliation: Byamukama, Denis. Makerere University. Department of Biochemistry; Uganda
Author affiliation: Coakley, Tricia. University Of Kentucky; Estados Unidos
Author affiliation: Ferguson, Christobel. ALS Water Sciences Group; Australia
Author affiliation: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; Etiopía
Author affiliation: Ko, GwangPyo. Seoul National University; Corea del Sur
Author affiliation: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países Bajos
Author affiliation: Mushi, Douglas. Sokoine University; Tanzania
Author affiliation: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Author affiliation: Pradhan, Bandana. Tribhuvan University; Nepal
Author affiliation: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Author affiliation: Schade, Margit A.. Bavarian Environment Agency; Alemania
Author affiliation: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; Austria
Author affiliation: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
Author affiliation: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; Hungría
Author affiliation: Vrajmasu, Virgil. Veterinary State Laboratory; Rumania
Author affiliation: Wuertz, Stefan. University Of California At Davis; Estados Unidos
Author affiliation: Mach, Robert L.. Vienna University of Technology; Austria
Author affiliation: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas