Abstract:

En los meses de julio de 2015 y enero de 2016 se colectaron garrapatas de un gato montés (Leopardus geoffroyi) localizado en la provincia de Buenos Aires y de un canino doméstico (Canis lupus familiaris) en la provincia de Santa Fe, respectivamente. El objetivo del presente trabajo es reportar el primer registro de Amblyomma aureolatum en la provincia de Buenos Aires y el primer hallazgo sobre caninos domésticos de la provincia de Santa Fe. Amblyomma aureolatum presenta importancia sanitaria ya que ha sido implicada como vector de Rickettsia rickettsii, uno de los agentes causales del grupo de las fiebres manchadas en humanos, y de Rangelia vitalii, agente causal de piroplasmosis en caninos domésticos, enfermedad vulgarmente conocida como “nambiuvú”. El presente reporte es un alerta para la comunidad sobre la presencia de A. aureolatum en nuevas localidades con el fin de implementar medidas profilácticas en caninos domésticos, para prevenir la infestación por este parásito y así evitar posibles patologías asociadas.

In July of 2015 and January of 2016, ticks were recovered from a Geoffroy’s cat (Leopardus geoffroyi) in Buenos Aires province and from a domestic dog (Canis lupus familiaris) in Santa Fe province, respectively. All ticks were identified as Amblyomma aureolatum. This report is the first record of A. aureolatum in Buenos Aires province and the first record in domestic dogs of Santa Fe province. Amblyomma aureolatum is a recognized vector of Rickettsia rickettsii, a spotted fever group agent in humans, and of Rangelia vitalii, a pathogenic piroplasmid of dogs, commonly referred to as “nambiuvú”. These findings allow local people to be aware of the presence of A. aureolatum to prevent infestations in dogs, and thus avoiding the occurrence of tick-borne diseases.

Author affiliation: Colombo, Valeria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina

Author affiliation: Pereira, Javier Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina

Author affiliation: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina

Author affiliation: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina

Abstract:

En los meses de julio de 2015 y enero de 2016 se colectaron garrapatas de un gato montés (Leopardus geoffroyi) localizado en la provincia de Buenos Aires y de un canino doméstico (Canis lupus familiaris) en la provincia de Santa Fe, respectivamente. El objetivo del presente trabajo es reportar el primer registro de Amblyomma aureolatum en la provincia de Buenos Aires y el primer hallazgo sobre caninos domésticos de la provincia de Santa Fe. Amblyomma aureolatum presenta importancia sanitaria ya que ha sido implicada como vector de Rickettsia rickettsii, uno de los agentes causales del grupo de las fiebres manchadas en humanos, y de Rangelia vitalii, agente causal de piroplasmosis en caninos domésticos, enfermedad vulgarmente conocida como “nambiuvú”. El presente reporte es un alerta para la comunidad sobre la presencia de A. aureolatum en nuevas localidades con el fin de implementar medidas profilácticas en caninos domésticos, para prevenir la infestación por este parásito y así evitar posibles patologías asociadas.

In July of 2015 and January of 2016, ticks were recovered from a Geoffroy’scat (Leopardus geoffroyi) in Buenos Aires province and from a domestic dog (Canis lupus familiaris) in Santa Fe province, respectively. All ticks were identified as Amblyommaaureolatum. This report is the first record of A. aureolatumin Buenos Aires province and the first record in domestic dogs of Santa Fe province. Amblyomma aureolatumis a recognized vector of Rickettsia rickettsii, a spotted fever group agent in humans, and of Rangelia vitalii, a pathogenic piroplasmid of dogs, commonly referred to as “nambiuvú”. These findings allow local people to be aware of the presence of A. aureolatumto prevent infestations in dogs, and thus avoiding the occurrence of tick-borne diseases

EEA Rafaela

Author affiliation: Colombo, Valeria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina

Author affiliation: Pereira, Javier Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; Argentina

Author affiliation: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina

Author affiliation: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina

Abstract:

The aim of this work was to determine the evolutionary relationship among tick populations of Rhipicephalus sanguineus sensu lato distributed in Africa north of the Sahara and different lineages of R. sanguineus s.l. distributed in different regions of Sub-Saharan Africa, America and Europe through the analysis of DNA sequences of two mitochondrial genes. One hundred and thirty six 16S rRNA gene sequences and twenty-seven 12S rRNA gene sequences of R. sanguineus s.l. were analyzed. Phylogenetic analyses were performed including different lineages of R. sanguineus s.l. from America, Europe and Africa, and species belonging to the R. sanguineus group as Rhipicephalus camicasi, Rhipicephalus guilhoni, Rhipicephalus sulcatus, Rhipicephalus rossicus, Rhipicephalus pusillus, Rhipicephalus turanicus and Rhipicephalus leporis. At least two different lineages of R. sanguineus s.l. are living in sympatry in Africa north of the Sahara. One of these mitochondrial lineages belongs to the same evolutionary entity that R. sanguineus s.l. from tropical areas of America, R. sanguineus s.l. from Sub-Saharan Africa, R. camicasi and R. guilhoni. The other mitochondrial lineage of R. sanguineus s.l. present in Africa north of the Sahara is phylogenetically associated to R. sanguineus s.l. ticks from southeastern Europe (Romania, Turkey and Greece). Both evolutionary entities are clearly different to the evolutionary entity formed by R. sanguineus s.l. from western Europe and temperate areas of America. Thus, the name R. sanguineus s.s. cannot be assigned to any of the two evolutionary entities present in Africa north of the Sahara. The taxonomic status of these taxa will remain unresolved until new lines of evidence become available to complement the current results based on mitochondrial DNA.

EEA Rafaela

Author affiliation: Chitimia-Dobler, Lidia. German Center of Infection Research. Bundeswehr Institute of Microbiology; Alemania

Author affiliation: Langguth, Johanna. University of Leipzig. Veterinary Faculty. Institute of Animal Hygiene and Veterinary Public Health; Alemania

Author affiliation: Pfeffer, Martin. University of Leipzig. Veterinary Faculty. Institute of Animal Hygiene and Veterinary Public Health; Alemania

Author affiliation: Kattner, Simonec. RWTH Aachen University, Aachen. Institute of Occupational and Social Medicine; Alemania

Author affiliation: Küpper, Thomas. RWTH Aachen University, Aachen. Institute of Occupational and Social Medicine; Alemania

Author affiliation: Friese, Daniela. German. Center of Infection Research. Bundeswehr Institute of Microbiology; Alemania

Author affiliation: Guglielmone, Alberto. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Author affiliation: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Abstract:

The aim of this work was to perform an analysis based on mtDNA sequences of the 16S rRNA gene in order to determine the phylogenetic position of ticks belonging to the Rhipicephalus sanguineus group from the Canary Islands, Cyprus, and Croatia. All the haplotypes obtained from ticks collected in the Canary Islands and Croatia grouped with R. sanguineus sensu stricto from France, Portugal, Italy, Switzerland, Argentina, Chile, Uruguay, and the USA. The sequences of R. sanguineus sensu lato from Cyprus formed a clade with R. sanguineus s.l. from Egypt, Turkey, and Romania, which belongs to the “Rhipicephalus sp. morphotype I” or “southeastern European lineage.” Ticks determined as R. turanicus s.l. from Cyprus clustered separately from the remaining clades of the R. sanguineus group, including R. turanicus s.s. The data show that R. sanguineus s.s. is present in the Canary Islands and Croatia, while R. sanguineus “southeastern lineage” is found in Cyprus.

EEA Rafaela

Author affiliation: Chitimia-Dobler, Lidia. German Center of Infection Research. Bundeswehr Institute of Microbiology; Alemania. University of Hohenheim; Alemania

Author affiliation: Kurzrock, Lina. University of Hohenheim; Alemania

Author affiliation: Molčányi, Tomáš. Eslovaquia. Armed Forces of Slovak Republic. Surgeon General Office; Eslovaquia

Author affiliation: Rieß, Ramona. German Center of Infection Research. Bundeswehr Institute of Microbiology; Alemania

Author affiliation: Mackenstedt, Ute. University of Hohenheim. Parasitology Unit; Alemania

Author affiliation: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Abstract:

The aim of this work was to describe two novel strains of Ehrlichia associated to Amblyomma tigrinum from Argentina. Molecular detection of agents belonging to the family Anaplasmataceae was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. The results have shown that two different strains of Ehrlichia sp. associated to A. tigrinum are circulating in peri-urban areas of Argentina. The Ehrlichia strain detected in ticks from San Luis Province, named as Ehrlichia sp. strain San Luis, is closely related to the Ehrlichia chaffeensis. The novel Ehrlichia strain detected in Córdoba Province, named as Ehrlichia sp. strain Córdoba, is phylogenetically related to three Ehrlichia strains from Brazil, two of them isolated from wild carnivorous and the third one isolated from horse. Even though Ehrlichia sp. strain Córdoba was clustered with the three Ehrlichia strains from Brazil, the genetic similarity was too low to consider them as the same taxonomic entity. Blood samples of dogs were positive to Anaplasma platys. The association of these two novel strains with A. tigrinum has epidemiological relevance because adult stages of this tick species are common parasite of dogs in rural and peri-urban areas and they are aggressive to humans. The presence of these two novel Ehrlichia strains implies a potential epidemiological risk in Argentina because the species of the genus Ehrlichia are known to be pathogenic to both domestic mammals and humans.

EEA Rafaela

Author affiliation: Cicuttin, Gabriel L. Buenos Aires (Argentina). Ministerio de Salud. Instituto de Zoonosis Luis Pasteur.; Argentina

Author affiliation: de Salvo, María Nazarena. Buenos Aires (Argentina). Ministerio de Salud. Instituto de Zoonosis Luis Pasteur.; Argentina

Author affiliation: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina