Authors: Palavecino Ruiz, Marcos Daniel; Ordóñez, Roxana Mabel; Isla, Maria Ines; Sayago, Jorge Esteban
Publication Date: 2016.
Language: English.
Abstract:
Fungal infections of fresh ripe Citrus limon fruit are the main cause of economiclosses during their commercialization. The aim of this work was to evaluate theactivity of the ethanolic extract (EE) from Parastrephia lepidophylla (Wedd.)Cabrera, a plant species that grows in the Argentine Northwest, onphytopatogenic fungi of lemon. Also, attempts were made to elucidate the modeof action of EE on the growth inhibition of phytopathogenic isolates of Penicillium digitatum Sacc. and Geotrichum citri-aurantii (Ferraris) E.E. Butler. The effect of the polyphenolic extracts on the conidia germination, mycelium growth and integrity of the plasma membrane was evaluated. The EE was active against both pathogens (minimal inhibitory concentration and minimal fungicidal concentration values of 150 μg of Galic Acid Equivalent/ml and 350 μg GAE/ml, respectively for both species). The conidia swelling and germination and the subsequent germ tube elongation was more affected by EE (100 % inhibition at 200 μg GAE/ml) than the vegetative body of the fungus (50 % inhibion at 400 μg GAE/ml). The fungus cell walls would not constitute a target for the EE components while the mechanism of action of the phytocomplex would be plasma membranes disruption. In vivo tests showed that P. leidophylla EE (700 μg GAE/ml) decreases the incidence of green mold disease in artificially inoculated lemons.
Author affiliation: Palavecino Ruiz, Marcos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Author affiliation: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Author affiliation: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Author affiliation: Sayago, Jorge Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Rodriguez, Ivana Fabiola; Sayago, Jorge E.; Torres, Sebastián; Zampini, Iris Catiana; Isla, Maria Ines; Ordóñez, Roxana Mabel
Publication Date: 2015.
Language: English.
Abstract:
A national program for citrus certification was started in Argentina in 2005 in order to provide healthy fruits free of toxic residues. In line with this goal, the aim of this study was to evaluate the efficacy of natural products, protein extracts obtained from potato tubers for the control of fungi responsible for disease in post-harvest citrus fruits. Different protein fractions were obtained from Solanum tuberosum tubers (IF25, IF50, SF25 and SF50) and their effect were evaluated on Penicillium digitatum and Geotrichum candidum, two citrus-pathogenic fungi. All fractions showed antifungal activity against both fungi species, the intensity of this activity being dependent on the type of fungus and extract. The fraction IF25 was the most active as an antifungal agent: it inhibited the mycelia growth of both pathogens, the elongation of the germ tube ofP. digitatum and the conidial isotropic growth of G. candidum as well as its polygalacturonase activity. None of the IF25 concentrations were mutagenic in Salmonella typhimurium TA98 or TA100 strains. The efficacy of protein extracts to control P. digitatum and G. candidum growth was tested in artificially inoculated citrus fruits. Extracts were tested built-in to the wax used in citrus industry to coat and protect the fruit. The IF25 extract was effective in inhibiting the growth and development of P.digitatum and G. candidum. Consequently, the IF25 extract plus the wax could be used preventively in controlling fungal infection on post-harvest citrus.
Author affiliation: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Author affiliation: Sayago, Jorge E.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Author affiliation: Torres, Sebastián. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Author affiliation: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Author affiliation: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Author affiliation: Ordóñez, Roxana Mabel. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas