Publication Date: 2008.
Background. Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipansis with genome types AA and BB respectively. Results. We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes), and resistance gene analogues. Conclusion. These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.
Author affiliation: Guimarães, Patricia M. Biotechnology Unit; Brasil
Author affiliation: Garsmeur, Olivier. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Author affiliation: Proite, Karina. Universidade do Brasília; Brasil. Biotechnology Unit; Brasil
Author affiliation: Leal Bertioli, Soraya C.M.. Biotechnology Unit; Brasil
Author affiliation: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Author affiliation: Chaine, Christian. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Author affiliation: Bertioli, David J. Genomic Sciences And Biotechnology Program; Brasil
Author affiliation: D'Hont, Angelique. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas