Authors: García Núñez, María Soledad; König, Guido Alberto; Berinstein, Analía; Carrillo, Elisa Cristina
Publication Date: 2010.
Language: English.
Abstract:
During the 2000-2001 epidemic of Foot-and-Mouth Disease Virus (FMDV) in Argentina, two FMDV serotype A viruses were identified among others. Since different pathogenic properties between these virus strains were noticed in cattle, we evaluated several biological properties and features of FMDV A/Arg/00 and FMDV A/Arg/01 in order to compare these viruses in terms of virulence and pathogenicity. Our results indicate that FMDV A/Arg/00 grows less efficiently than FMDV A/Arg/01, exemplified by smaller sized plaques, retarded one-step growth curves and overall low viral yields. Also, FMDV A/Arg/00 displayed the lowest specific infectivity in suckling mice requiring 50-fold more infectious particles than FMDV A/Arg/01 to generate a LD50 in suckling mice. Finally, FMDV A/Arg/00 did not cause death in adult C57Bl/6 mice even at high doses (107-106 PFU) whereas FMDV A/Arg/01 resulted lethal in doses as low as 102 PFU. Overall, we were able to demonstrate that these virus strains differ from each other in terms of virulence and pathogenicity.
Author affiliation: García Núñez, María Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Author affiliation: König, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Author affiliation: Berinstein, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Author affiliation: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Sansone, Gabriela; Calvente, Viviana; Benuzzi, Delia Aurora; Salinas, Eloy; Raba, Julio; Sanz Ferramola, Maria Isabel
Publication Date: 2014.
Language: English.
Abstract:
Objective: To study the effect of temperature on the morphological characteristics of Botrytis cinerea (B. cinerea) and its correlated with the genetic variability. B. cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries. Methods: Six strains from different host collected have been isolated and characterized by several methods as mycelial growth, fungicide resistance, pathogenicity and the effects of the temperature. Also was analyzed by PCR and distinguished by the presence or absence of transposable elements. Results: Results showed that clear morphological differences exist between strains at the temperature of 4, 12 and 28 °C. All strains analyzed molecularly were classified as Group II (transposa-type). Demonstrating a negative correlation between mycelial growth and other characteristics as the fungicide resistance and pathogenicity. Lastly, it is difficult to establish relationships phenotypic and genotypic between strains of B. cinerea. Conclusions: The results indicated that the mycelial growth, resistance at fungicide and pathogenicity are independent of the characteristics molecular, however, are dependent of a factor such as temperature.
Author affiliation: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Author affiliation: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Author affiliation: Sansone, Gabriela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Author affiliation: Calvente, Viviana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Author affiliation: Benuzzi, Delia Aurora. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Author affiliation: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Author affiliation: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Author affiliation: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Zanuzzi, Carolina Natalia; Scrochi, Mariela Rita; Fuentealba, Nadia Analia; Nishida, Fabian; Portiansky, Enrique Leo; Muglia, Cecilia Isabel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Galosi, Cecilia Monica
Publication Date: 2014.
Language: English.
Abstract:
Here, we used a murine model to describe and compare the pathogenic potential of the Argentinean equid herpesvirus 1 (EHV-1) AR8 strain with the Japanese HH1 reference strain. In AR8-inoculated animals, clinical signs began earlier, but the viremic phase was shorter. Virus isolation and DNA detection in the lungs, liver and spleen were positive for both strains at different times postinfection (pi). Infection foci produced by both strains were immunohistochemically detected in lungs from day 1 to day 4 pi. We conclude that whichever EHV-1 strain is selected to experimentally reproduce the disease, it needs appropriate standardization in order to provide valid conclusions.
Author affiliation: Zanuzzi, Carolina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Author affiliation: Scrochi, Mariela Rita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Fuentealba, Nadia Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Nishida, Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Author affiliation: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Author affiliation: Muglia, Cecilia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Author affiliation: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Author affiliation: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina
Author affiliation: Galosi, Cecilia Monica. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Zanuzzi, Carolina Natalia; Scrochi, Mariela Rita; Fuentealba, Nadia Analia; Nishida, Fabian; Portiansky, Enrique Leo; Muglia, Cecilia Isabel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Galosi, Cecilia Monica
Publication Date: 2014.
Language: English.
Abstract:
Here, we used a murine model to describe and compare the pathogenic potential of the Argentinean equid herpesvirus 1 (EHV-1) AR8 strain with the Japanese HH1 reference strain. In AR8-inoculated animals, clinical signs began earlier, but the viremic phase was shorter. Virus isolation and DNA detection in the lungs, liver and spleen were positive for both strains at different times postinfection (pi). Infection foci produced by both strains were immunohistochemically detected in lungs from day 1 to day 4 pi. We conclude that whichever EHV-1 strain is selected to experimentally reproduce the disease, it needs appropriate standardization in order to provide valid conclusions.
Author affiliation: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Scrochi, Mariela Rita. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Fuentealba, Nadia Analia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Muglia, Cecilia Isabel. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Zanuzzi, Carolina Natalia; Scrochi, Mariela Rita; Fuentealba, Nadia Analia; Nishida, Fabian; Portiansky, Enrique Leo; Muglia, Cecilia Isabel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Galosi, Cecilia Monica
Publication Date: 2014.
Language: English.
Abstract:
Here, we used a murine model to describe and compare the pathogenic potential of the Argentinean equid herpesvirus 1 (EHV-1) AR8 strain with the Japanese HH1 reference strain. In AR8-inoculated animals, clinical signs began earlier, but the viremic phase was shorter. Virus isolation and DNA detection in the lungs, liver and spleen were positive for both strains at different times postinfection (pi). Infection foci produced by both strains were immunohistochemically detected in lungs from day 1 to day 4 pi. We conclude that whichever EHV-1 strain is selected to experimentally reproduce the disease, it needs appropriate standardization in order to provide valid conclusions.
Author affiliation: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Scrochi, Mariela Rita. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Fuentealba, Nadia Analia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Muglia, Cecilia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Author affiliation: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Cobo, Eduardo Ruben; Canton, German; Morrell, Eleonora Lidia; Cano, Dora; Campero, Carlos Manuel
Publication Date: 2004.
Language: English.
Abstract:
Experimental infection of the reproductive tracts of heifers and bulls with Tetratrichomonas sp. isolated from preputial smegma of virgin bulls was attempted. Nine heifers and four bulls were challenged by inoculation of 7×106 Tetratrichomonas sp. into the vaginal lumen and preputial cavity, respectively. Vaginal mucus and preputial smegma samples were collected and cultured for Tetratrichomonas sp. Heifers were slaughtered in groups of three at 2, 9 and 21 days after inoculation. Two heifers and two bulls infected with Tritrichomonas foetus and two uninfected heifers were used as controls for the model infection. Tetratrichomonas sp. were only isolated in vaginal mucus of 7/9 inoculated heifers at 6 h post-inoculation, and genital secretions taken at slaughter time from vagina, uterus and oviduct were cultural negative. Bulls challenged with Tetratrichomonas sp. remained cultural negative. Since Tetratrichomonas sp. survived only a few hours in the female genitalia and did not survive in the male genitalia after experimental challenge, Tetratrichomonas sp. did not colonize the genital tract. These were likely trichomonads from the digestive tract. Collection of clean samples without fecal contamination from the reproductive tract is proposed as a measure to avoid Tetratrichomonas sp. transitory genital infection.
Author affiliation: Cobo, Eduardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Canton, German. Instituto Nacional de Tecnología Agropecuaria; Argentina
Author affiliation: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; Argentina
Author affiliation: Cano, Dora. Instituto Nacional de Tecnología Agropecuaria; Argentina
Author affiliation: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Publication Date: 2015.
Language: English.
Abstract:
Wheat blast (WB), caused by the fungus Magnaporthe oryzae B.C. Couch (anamorph Pyricularia oryzae Cavara), has caused up to 40 to 100% crop loss in South America (Kohli et al. 2011). WB was first detected in Argentina during 2007 in a limited area in the northern region of the country (Cabrera and Gutierrez 2007). During the 2012 growing season, M. grisea was found in wheat plants belonging to an experimental assay at IFSC, Lavallol, Lomas de Zamora, Buenos Aires Province, Argentina (Perelló et al. 2013). No additional instances of the disease were found in neighboring fields. Examination of diseased plants showed the presence of elliptical, grayish to tan necrotic lesions with dark borders on the leaf. On spikes, blackening of the rachis and partial bleaching was observed. Incubation of these plants in a moist chamber for 12 h led to the production of pyriform conidia from these lesions typical of Pyricularia-like fungus. Single-spore isolates of the fungus were obtained by transferring conidia to acidified 1.5% water agar and then transferring single, germinated conidia to V8 juice agar and OMA medium. For long term preservation, fungal material was grown and kept on dry filter paper in polypropylene tubes. M. oryzae was also successfully isolated during 2013 from native weeds and grasses (Stenotaphrum secundatum, Setaria sp., Eleusine sp., Bromus unioloides, Lolium perenne, Echinochloa crus-galli, Avena fatua, and Cynodon dactylon) growing adjacent to wheat and rice fields belonging to the Experimental Station Julio Hirschhorn, Los Hornos-FCAyF, UNLP. Pathogenicity tests were conducted using 10 isolates from wheat and five isolates from grasses. Fungal suspensions (1 × 105 conidia per ml) were spray-inoculated on wheat seedling (Zadoks’ growth stage 14) and at heading (Zadoks’ growth stage 61) on cultivars BioINTA 3004 and Baguette 18 (Zadoks et al. 1974). Plants were incubated in a moist chamber at 22°C and 100% RH for 48 h. Disease severity was estimated in each strain-wheat cultivar combination at 10, 13, and 17 days after inoculation. Symptoms were similar to those observed in the field, while the control treatment remained symptomless. Leaf infection ranged from 2.7 to 64.3% and different phenotypic reactions were observed such as pinhead-sized spots; small or enlarged brown lesions with no distinguishable center; and the typical blast lesions, elliptical or roughly diamond-shaped with gray centers and a thin, brown border. On spikes, partial or total bleaching of spikes was observed up to 50% severity. Necrosis of the glumes and rachis was occasionally observed. Thousand seed weight was significantly reduced by fungal infection (63.1%). Reduction of grain germination (43.6%), seed rot, and seedling rot and blight were also observed after in vitro inoculations of grain. The pathogen was reisolated from lesions using the methods previous described, confirming Koch’s postulates. Morphobiometrical characteristics of the typical pyriform (pear-shaped) hyaline 2-septate conidia of the recovered isolates were in agreement with the identification of the fungus as M. oryzae (Subramanian 1968). A culture specimen was deposited in the IFSC herbarium (No. PGW0812). Our results indicate that M. oryzae has established itself in the principle wheat agroecological region of Buenos Aires, Argentina, and may pose a significant threat to wheat production, and therefore poses a significant threat to wheat production in Argentina.
Author affiliation: Perello, Analia Edith. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Author affiliation: Martinez, I.. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Author affiliation: Molina, María del Carmen. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Instituto Fitotécnico de ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Alberione, E.; Ortega, Leonel Maximilano; Astoreca, Andrea Luciana; Salines, Nicolas; Alconada Magliano, Teresa Maria
Publication Date: 2016.
Language: Spanish.
Abstract:
Fusarium graminearum es el principal agente causal de fusariosis de la espiga (FET) de trigo en Argentina, disminuye el rendimiento, reduce la calidad del grano y su uso final, y libera micotoxinas. El objetivo de este estudio fue identificar nuevas fuentes de resistencia a FET en cultivares y líneas de trigo argentinos al comparar con cultivares resistentes internacionales conocidos. El comportamiento de Triticum aestivum se evaluó frente a F. graminearum como indicador de resistencia a la penetración de FET a la espiga de trigo (resistencia de tipo I) en. Los ensayos se realizaron en condiciones de campo en Marcos Juárez (62° 6’ 7” O, 32° 41’ 39” S; 116 msnm), provincia de Córdoba, Argentina, durante la primavera de 2010, con la humedad controlada por un sistema de microaspersión. Para este estudio distribuimos 210 variedades de trigo con espigas inoculadas por pulverización a mano sobre una parcela colina, que fue la unidad experimental; las variedades de trigo se plantaron e inocularon por duplicado. El diseño experimental fue de bloques completos aleatorios y los resultados fueron analizados por ANOVA y el test de Fisher-LSD (p£0.05) para comparar los valores medios de incidencia y severidad entre los tratamientos. La resistencia se evaluó a través de criterios patométricos y de rendimiento. La alta infectividad del aislamiento de F. graminearum permitió evaluar la enfermedad en todas las variedades de trigo. Todas las variables, excepto la incidencia, fueron estadísticamente diferentes entre los cultivares de trigo de Argentina, mientras que sólo el peso de mil granos difirió significativamente en los cultivares internacionales. De los 92 genotipos de trigo de línea avanzada de tres diferentes ciclos de crecimiento, 57 % fueron moderadamente resistentes y 40 % moderadamente susceptibles a infecciones. La resistencia a FET evaluada en todos los cultivares y líneas de genotipos nacionales e internacionales permitió identificar variedades de cinco niveles de resistencia a enfermedades. El conocimiento del comportamiento genético puede contribuir al desarrollo de nuevos cruzamientos para generar variedades de trigo con resistencia mejorada contra FET.
The aim of this manuscript was to identify new sources of resistance to Fusarium head blight (FHB) in Argentinean wheat cultivars and lines, compared to known resistant international cultivars. For that purpose, the behavior against Fusarium graminearum as estimative of type I resistance or resistance to penetration of the pathogen in the wheat spike (Triticum aestivum) was evaluated. The assays were carried out under field conditions in Marcos Juárez location, Córdoba (Argentina) during 2010, with partial control of humidity and temperature, by artificial inoculation of wheat spikes. The assays were sown according to a statistical design of randomized complete blocks with two repetitions. Statistical analyzes were performed using ANOVA; means of incidence and severity variables were compared by Fisher LSD test to determine the significant differences among the different treatments. Resistance was evaluated through pathometrics and yield variables. The F. graminearum isolate used was highly infective and allowed us to evaluate the disease in 210 genotypes. There were statistical differences (p ≤ 0.05) in all variables except for incidence. However, in the international cultivars, significant differences (p ≤ 0.05) were found only for the variable ?thousand grains weight?. In another set of genotypes, integrated by advanced wheat lines of different maturity dates, the behavior to disease can be considered good due to the fact that many of the lines showed moderately resistance (57 %) and moderately susceptible (40 %). Evaluation of resistance to FHB of the all genotypes (cultivars and national and international lines) allowed identifying different levels of disease resistance. Therefore, knowledge of the genetic behavior of resistance of the evaluated germoplasm, characterized by wide genetic variability, could be considered as new possible genetic contributions to be used in new crosses to ensure the improved varieties against Fusarium head blight.
Author affiliation: Alberione, E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Author affiliation: Ortega, Leonel Maximilano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Author affiliation: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Author affiliation: Salines, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Author affiliation: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Mateu, Cecilia Gabriela; Artuso, María Carolina; Pujol, Carlos Alberto; Linero, Florencia Natalia; Scolaro, Luis Alberto; Carlucci, Maria Josefina
Publication Date: 2017.
Language: English.
Abstract:
Natural polysaccharides known as carrageenans are potent and selective inhibitors of herpes simplex virus through blocking the interaction of the virus with the cellular receptor. Passaging the virus in vitro in the presence of carrageenans types k and ι enabled us to generate variants of herpes simplex type 2. At passage 22, four clones were selected for further characterization: ι22-9, ι22-12, k22-12 and k22-13. Variants showed a syncytial phenotype, grew at similar titers when compared to parental virus and exhibited moderate resistance to carrageenans. These were found to have a mutation in the thymidine kinase gene in the case of k22-13 (aa 149 Val to Ala) and in the DNA pol gene in the case of ι22-12 (aa 789 Met to Thr). In variant k22-12, three substitutions in the DNA pol gene were identified. Variants were less virulent than parental strain when tested intravaginally or intranasally in mice. Attenuation correlated with higher levels of IL-6 and TNF-α in animals inoculated with the variants. Selective pressure on the external glycoproteins of the virus may generate viruses with alterations in genes unrelated to the target of action of the selection agent. Study of this type of variation might help us to understand the basis of persistent viral strategies, which in turn may play a role in the development of virus-host symbioses.
Author affiliation: Mateu, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Author affiliation: Artuso, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Author affiliation: Pujol, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Author affiliation: Linero, Florencia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Author affiliation: Scolaro, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Author affiliation: Carlucci, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Publication Date: 2018.
Language: English.
Abstract:
Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens associated with outbreaks and hemolytic-uremic syndrome. Cattle and meat foods are the main reservoir and infection source, respectively. Pathogenicity islands (PAIs) play an important role in STEC pathogenicity, and non-locus of the enterocyte effacement(LEE) effector (nle) genes present on them encode translocated substrates of the type III secretion system. A molecular risk assessment based on the evaluation of the nle content has been used to predict which STEC strains pose a risk to humans. The goal was to investigate the distribution of the PAIs OI (O-island)-36 (nleB2, nleC, nleH1-1, nleD), OI-57 (nleG2-3, nleG5-2, nleG6-2), OI-71 (nleA, nleF, nleG, nleG2-1, nleG9, nleH1-2) and OI-122 (ent/espL2, nleB, nleE, Z4321, Z4326, Z4332, Z4333) among 204 clinical, food and animal isolates belonging to 52 non-O157:H7 serotypes. Differences in the frequencies of genetic markers and a wide spectrum of PAI virulence profiles were found. In most LEE-negative strains, only module 1 (Z4321) of OI-122 was present. However, some unusual eae-negative strains were detected, which carried other PAI genes. The cluster analysis, excluding isolates that presented no genes, defined two major groups: eae-negative (determined as seropathotypes (SPTs) D, E or without determination, isolated from cattle or food) and eae-positive (mostly identified as SPTs B, C, or not determined).
Author affiliation: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Author affiliation: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Author affiliation: González, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Author affiliation: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas