Publication Date: 2017.
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Author affiliation: Craig, María Isabel. INTA. Instituto de Virología; Argentina
Author affiliation: Rojas, María Florencia. INTA. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Author affiliation: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina
Author affiliation: Olivera, Valeria Soledad. INTA. Instituto de Virología; Argentina
Author affiliation: Vagnozzi, Ariel Eduardo. INTA. Instituto de Virología; Argentina
Author affiliation: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos
Author affiliation: Konig, Guido Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Keywords: Laringotraqueitis; Virus Bronquitis Infecciosa Aviar; Virus de los Animales; Epidemiología; Glicoproteínas; Genética Molecular; Molecular Genetics; Glycoproteins; Epidemiology; Animal Viruses; Avian Infectious Bronchitis Virus; Laryngotracheitis; Infectious Laryngotracheitis Virus; Spatial Cluster Analysis; Análisis de Cluster Espacial; Argentina.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria