Publication Date: 2018.
Lamanema chavezi (Family Molineidae) is a parasitic nematode of South American camelids (SACs). A few studies have reported this parasite in SACs, mainly in domestic camelid species (llama and alpaca). Parasite identification by means of copro-parasitological methods is non-invasive and allows performing epidemiological studies. However, egg misidentification and difficulty to culture third-stage larvae do not allow identifying nematodes to species level. In contrast, molecular tools allow identifying eggs of gastrointestinal nematodes more accurately. However, the little genomic information available in databases for some species prevents an accurate diagnosis. In the present work, L. chavezi females present in feces of llamas from northwestern Argentina were molecularly characterized to obtain genomic information and improve parasitological diagnosis of L. chavezi-like eggs present in guanaco feces from southeastern Argentina. An 833-bp fragment of nuclear ribosomal DNA (rDNA) and a 434-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene from both L. chavezi females and eggs were amplified and sequenced. Comparison between sequences from females and eggs showed 99–99.6% identity to rDNA and 99.5–96.1% to the cox1 gene fragments, confirming egg morphological assignment. A higher divergence between sequences was observed in the cox1 fragment, with a maximum variation of 3.9%. The examination of eggs found in guanaco feces from southeastern Argentina and their specific molecular identification represent the first record for this host in Argentine Patagonia and contribute to improving the diagnosis of gastrointestinal nematodes in SACs, mainly in wild camelids.
Instituto de Investigación Animal Chaco Semiarido
Author affiliation: Petrigh, Romina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Zoonosis Parasitarias; Argentina
Author affiliation: Cafrune Wierna, María Mercedes. INTA. Instituto de Investigación Animal Chaco Semiarido. Area de Investigación en Salud Animal; Argentina
Author affiliation: Fugassa, Martín Horacio Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Zoonosis Parasitarias; Argentina
Keywords: Secuencia de ADN; ADN Mitocondrial; Guanaco; Genética Molecular; Molineidae; Conservación de la Naturaleza; ADN Ribosomal; DNA Sequence; Mitochondrial DNA; Molecular Genetics; Nature Conservation; Ribosomal DNA; Lamanema Chavezi; Lama Guanicoe; Región Patagónica; Molecular Identification; Molineid Eggs; Wildlife Conservation.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Publication Date: 2013.
Native-European contact in Tierra del Fuego was a rapid process, for which little ethnographic information has been produced. Although the Fuegian dog seemed to have been important to Selk’nam people’s life, the taxonomic status of this extinct animal is still uncertain. The aim of the present work was to determine the zoological identity of a taxidermized canid belonging to a Fagnano Regional Museum collection, Río Grande, Tierra del Fuego. For this purpose, DNA from Fuegian dog and patagonian wild canids (Lycalopex culpaeus, Lycalopex griseus and Lycalopex gymnocercus) hairs was extracted. An mtDNA Region Control fragment was amplified by PCR and sequenced. Sequence alignment was performed among the sequences that were obtained in this research and the Canis lupus familiaris sequence from GenBank. Pairwise analysis showed a higher identity between the Fuegian dog and the culpeo fox (97.57%), with greater divergence with the current domestic dog (88.93%). These results were supported by the molecular phylogenetic analysis, suggesting atypical fox domestication by hunter-gatherers.
Author affiliation: Petrigh, Romina Sandra. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Fugassa, Martín Horacio. Universidad Nacional de Mar del Plata; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas