Publication Date: 2017.
Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.
Author affiliation: Ke, Zhonghe. University of Rochester; Estados Unidos
Author affiliation: Mallik, Pramit. University of Rochester; Estados Unidos
Author affiliation: Johnson, Adam B.. University of Rochester; Estados Unidos
Author affiliation: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Author affiliation: Nevo, Eviatar. University of Haifa; Israel
Author affiliation: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados Unidos
Author affiliation: Gladyshev, Vadim N.. Harvard Medical School; Estados Unidos
Author affiliation: Seluanov, Andrei. University of Rochester; Estados Unidos
Author affiliation: Gorbunova, Vera. University of Rochester; Estados Unidos
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas