Authors: Tirado González, Irene; Freitag, Nancy; Barrientos, Gabriela Laura; Shaikly, Valerie; Nagaeva, Olga; Strand, Magnus; Kjellberg, Lennart; Klapp, Burghard F.; Mincheva Nilsson, Lucia; Cohen, Marie; Blois, Sandra M.
Publication Date: 2013.
Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLAG) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.
Author affiliation: Tirado González, Irene. Medicine University Berlin; Alemania
Author affiliation: Freitag, Nancy. Medicine University Berlin; Alemania
Author affiliation: Barrientos, Gabriela Laura. Medicine University Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Shaikly, Valerie. University Of Essex; Reino Unido. Assisted Reproduction and Gynaecology Centre; Reino Unido
Author affiliation: Nagaeva, Olga. Universidad de Umea; Suecia
Author affiliation: Strand, Magnus. Universidad de Umea; Suecia
Author affiliation: Kjellberg, Lennart. Universidad de Umea; Suecia
Author affiliation: Klapp, Burghard F.. Medicine University Berlin; Alemania
Author affiliation: Mincheva Nilsson, Lucia. Universidad de Umea; Suecia
Author affiliation: Cohen, Marie. Laboratoire d’Hormonologie; Suiza
Author affiliation: Blois, Sandra M.. Medicine University Berlin; Alemania
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas