Authors: Benintende, Graciela Beatriz; Berretta, Marcelo Facundo; Zandomeni, Ruben; Sauka, Diego Hernan; Amadio, Ariel F.; Ortiz, Elio Maximiliano; Navas, Laura Emilce
Publication Date: 2017.
Language: English.
Abstract:
We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.
EEA Rafaela
Author affiliation: Benintende, Graciela Beatriz. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina
Author affiliation: Berretta, Marcelo Facundo. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Zandomeni, Rubén. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Sauka, Diego Hernan. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Amadio, Ariel. INTA. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Ortiz, Elio Maximiliano. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Navas, Laura Emilce. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Keywords: Genética; Bacillus Thuringiensis; Genes; Genetics; pFR260; Genes Insecticidas.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria