Publication Date: 2010.
Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.
Author affiliation: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; Argentina
Author affiliation: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Author affiliation: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Author affiliation: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Author affiliation: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Author affiliation: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; Argentina
Author affiliation: Storey, Bayard T.. University of Pennsylvania; Estados Unidos
Author affiliation: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; España
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas