Authors: Carabajal Paladino, Leonale Suzel; Muntaabski, Irina; Lanzavecchia, Silvia Beatriz; Le Bagousse-Pinguet, Yoann; Viscarret, Mariana Mabel; Juri, Marianela Lucía; Fueyo Sánchez, Luciana; Papeschi, Alba Graciela; Cladera, Jorge Luis; Bressa, María José
Publication Date: 2015.
Language: English.
Abstract:
We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general.
Instituto de Genética
Author affiliation: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. INTA. Instituto de Genética; Argentina
Author affiliation: Muntaabski, Irina INTA. Instituto de Genética; Argentina
Author affiliation: Lanzavecchia, Silvia Beatriz. INTA. Instituto de Genética; Argentina
Author affiliation: Le Bagousse-Pinguet, Yoann. University of South Bohemia. Department of Botany; República Checa
Author affiliation: Viscarret, Mariana Mabel. INTA. Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina
Author affiliation: Juri, Marianela Lucía. INTA. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Fueyo Sánchez, Luciana. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Author affiliation: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética; Argentina
Author affiliation: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Suárez, Lorena; Buonocore Biancheri, María Josefina; Sánchez, Guillermo; Murúa, Fernando; Funes, Claudia Fernanda; Kirschbaum, Daniel Santiago; Molina, Diego; Laría, Osvaldo; Ovruski Alderete, Sergio Marcelo
Publication Date: 2019.
Language: English.
Abstract:
Exotic plants favor persistence and spread of the invasive medfly, Ceratitis capitata. Peach and orange are key host plants for medfly proliferation in Argentina. Consequently, actions to suppress medfly populations are taken, especially those performing augmentative releases of parasitoids. This study provides information on the capability of two population lines of the parasitoid Diachasmimorpha longicaudata to control medfly infesting the fruits of peach, as well as sour and sweet orange. One parasitoid line comes from non-irradiated larvae of wild medfly. The other comes from irradiated larvae of the Temperature Sensitive Lethal Vienna-8 medfly strain. The parasitoid host-finding ability in each aforementioned fruit species, the effectiveness of females to kill medfly larvae, the fruit height level preference for parasitoid foraging activity, and the influence of environmental conditions on parasitoid performance were compared and assessed. Parasitoids foraged for 48 h on fruits artificially inoculated with wild medfly larvae in field cages. Females of both parasitoid lines showed a similar effectiveness pattern, foraged efficiently on fruit at ground and canopy levels, and were able to overcome local climate conditions and to develop at least one new generation under natural environmental conditions. These outcomes may provide relevant information for the implementation of augmentative biological control against medfly.
EEA Famaillá
Author affiliation: Suárez, Lorena. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Programa de Control y Erradicación de Mosca de los Frutos; Argentina. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Dirección de Sanidad Vegetal, Animal y Alimentos; Argentina
Author affiliation: Buonocore Biancheri, María Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Author affiliation: Sánchez, Guillermo. Universidad Nacional de San Juan. Departamento de Biología. Diversidad de Invertebrados; Argentina
Author affiliation: Murúa, Fernando. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Programa de Control y Erradicación de Mosca de los Frutos; Argentina. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Dirección de Sanidad Vegetal, Animal y Alimentos; Argentina. Universidad Nacional de San Juan. Departamento de Biología. Diversidad de Invertebrados; Argentina
Author affiliation: Funes, Claudia Fernanda. INTA. Estación Experimental Agropecuaria Famaillá; Argentina
Author affiliation: Kirschbaum, Daniel Santiago. INTA. Estación Experimental Agropecuaria Famaillá; Argentina
Author affiliation: Molina, Diego. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Dirección de Sanidad Vegetal, Animal y Alimentos; Argentina
Author affiliation: Laría, Osvaldo. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Programa de Control y Erradicación de Mosca de los Frutos; Argentina. Provincia de San Juan. Ministerio de Producción y Desarrollo Económico. Secretaria de Agricultura, Ganadería y Agroindustria. Dirección de Sanidad Vegetal, Animal y Alimentos; Argentina
Author affiliation: Ovruski Alderete, Sergio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Devescovi, Francisco; Bachmann, Guillermo Enrique; Nussenbaum, Ana Laura; Viscarret, Mariana Mabel; Cladera, Jorge Luis; Segura, Diego Fernando
Publication Date: 2017.
Language: English.
Abstract:
The optimal use of available host by parasitoid insects should be favoured by natural selection. For solitary parasitoids, superparasitism (i.e. the egg-laying of several eggs/host) may represent a detrimental phenomenon both in a biological and an applied sense, but under certain circumstances it may be adaptive. Here, we studied the effects of increasing levels of superparasitism (LSPs, number of parasitoid larvae/host) on fitness-related parameters of the immature and adult stages of Diachasmimorpha longicaudata, a solitary endoparasitoid parasitizing Ceratitis capitata. We investigated the moment when supernumerary parasitoid larvae are eliminated and the effects produced by this process, together with its repercussion on female fecundity, parasitism rate, sex ratio, adult survival, flight ability and body size. Complete elimination of competitors occurred soon after larval hatching, before reaching the second larval stage. Elimination process took longer at higher LSPs, although a normal developmental (egg–adult) time was achieved. For LSPs 1, 2, 3 and 5 the effects on parasitoid emergence were mild, but LSP 10 led to the death of all developing parasitoids. Aside from this, to develop in superparasitized hosts did not significantly affect any of the evaluated parameters, and only a female-biased sex ratio was observed at higher LSPs. However, the effects of superparasitism on the adults may have a different outcome under more variable conditions in the field, once they are released for biological control purposes.
Inst. de Microbiología y Zoología Agrícola IMyZA
Author affiliation: Devescovi, Francisco. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Author affiliation: Bachmann, Guillermo Enrique. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Author affiliation: Nussenbaum, Ana Laura. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Author affiliation: Viscarret, Mariana Mabel. INTA. Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones Lucha Biológica; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Author affiliation: Segura, Diego Fernando. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Carabajal Paladino, Leonale Suzel
Publication Date: 2011.
Language: Spanish.
Abstract:
Tesis para obtener el grado de Doctora en el área de Ciencias Biológicas, de la Universidad de Buenos Aires, en 2011
El parasitoide bracónido Diachasmimorpha longicaudata es ampliamente utilizado como controlador biológico de moscas de los frutos; sin embargo, es escaso el conocimiento disponible sobre su genética. A fin de aportar información y mejorar su cría masiva, se estudió su sistema de determinación del sexo mediante la realización de cruzamientos de alta y baja endogamia, utilizando herramientas de citogenética para determinar el nivel de ploidía de la descendencia. Asimismo, se analizó su espermatogénesis y su cariotipo en detalle, realizando aportes a la Teoría de Interacción Mínima, desarrollada para el estudio de la evolución del cariotipo en Hymenoptera. En este parasitoide el sexo se determina por haplodiploidía (machos haploides, hembras diploides), pero en condiciones de alta endogamia ocurre un sesgo significativo de la proporción de sexos hacia machos, debido a la generación de machos diploides. El sexo estaría determinado por el estado de un promedio de tres loci no ligados. Los individuos homocigotas para los loci sexuales se desarrollan en machos diploides. Esta información permitirá mejorar los protocolos de cría masiva de la especie, tratando de minimizar el nivel de endogamia a fin de evitar un incremento en la producción de machos. El estudio citogenético permitió corroborar el número cromosómico descripto previamente. Se analizó la cantidad, composición y distribución de la heterocromatina constitutiva, se mapearon los “clusters” de genes ribosomales y se determinó la cantidad de “clusters” activos. La información obtenida permitió aportar más evidencias a favor de la Teoría de Interacción Mínima.
Diachasmimorpha longicaudata is a braconid parasitoid widely used as biological control agent against fruit flies. In spite of its importance, little is known about its genetics. Different aspects were studied in order to provide more information and to optimize its massive production. Its sex determination system was analyzed using inbred and outbred crosses, and cytogenetic tools to analyze the ploidy level of the descendants. Also its spermatogenesis and karyotype were analyzed in detail, contributing to the Minimum Interaction Theory proposed for the study of karyotype evolution in Hymenoptera. Sex is determined by haplodiploidy (males are haploid, females are diploid), but under inbreeding a significant bias towards male progeny is observed due to the generation of diploid males. Sex is presumably determined by a mean of three independent loci. Homozigotes for the sex loci develop into diploid males. In order to avoid an increment in male production, this information would be useful to improve the protocols of massive production of this species, regarding to minimizing the inbreeding levels. The cytogenetic study allowed the corroboration of the chromosome number of the species. The amount, composition and distribution of constitutive heterochromatin were analyzed. The ribosomal gene clusters were located and the quantity of active sites was determined. The obtained information gave more evidence in favor of the Minimum Interaction Theory.
Instituto de Genética
Author affiliation: Carabajal Paladino, Leonale Suzel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Segura, Diego Fernando; Nussenbaum, Ana Laura; Viscarret, Mariana Mabel; Devescovi, Francisco; Bachmann, Guillermo Enrique; Corley, Juan Carlos; Ovruski Alderete, Sergio Marcelo; Cladera, Jorge Luis
Publication Date: 2016.
Language: English.
Abstract:
Parasitoids searching for polyphagous herbivores can find their hosts in a variety of habitats. Under this scenario, chemical cues from the host habitat (not related to the host) represent poor indicators of host location. Hence, it is unlikely that naïve females show a strong response to host habitat cues, which would become important only if the parasitoids learn to associate such cues to the host presence. This concept does not consider that habitats can vary in profitability or host nutritional quality, which according to the optimal foraging theory and the preference-performance hypothesis (respectively) could shape the way in which parasitoids make use of chemical cues from the host habitat. We assessed innate preference in the fruit fly parasitoid Diachasmimorpha longicaudata among chemical cues from four host habitats (apple, fig, orange and peach) using a Y-tube olfactometer. Contrary to what was predicted, we found a hierarchic pattern of preference. The parasitism rate realized on these fruit species and the weight of the host correlates positively, to some extent, with the preference pattern, whereas preference did not correlate with survival and fecundity of the progeny. As expected for a parasitoid foraging for generalist hosts, habitat preference changed markedly depending on their previous experience and the abundance of hosts. These findings suggest that the pattern of preference for host habitats is attributable to differences in encounter rate and host quality. Host habitat preference seems to be, however, quite plastic and easily modified according to the information obtained during foraging.
Inst. de Microbiología y Zoología Agrícola IMyZA
Author affiliation: Segura, Diego Fernando. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Nussenbaum, Ana Laura. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Viscarret, Mariana Mabel. INTA. Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina
Author affiliation: Devescovi, Francisco. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Bachmann, Guillermo Enrique. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Corley, Juan Carlos. INTA. Estación Experimental Agropecuaria Bariloche. Grupo de Ecología de Poblaciones de Insectos; Argentina
Author affiliation: Ovruski, Sergio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Segura, Diego Fernando; Viscarret, Mariana Mabel; Ovruski Alderete, Sergio Marcelo; Cladera, Jorge Luis
Publication Date: 2012.
Language: English.
Abstract:
Chemical information is crucial to insect parasitoids for successful host location. Here, we evaluated the innate response of Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae), a fruit fly larval parasitoid, to cues from host and host habitat (i.e., fruit infested with host larvae). We first assessed the preference of female parasitoids between oranges infested with Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) and non‐infested fruit. Females were highly attracted towards infested oranges on the basis of volatile chemical cues. After this initial experiment, we aimed at revealing the potential sources of volatile cues present in an infested fruit. To this end, we considered five potential sources: (1) punctured fruit; (2) fly feeding, frass, or host‐marking pheromone deposited on the orange surface; (3) larval activity inside the fruit; (4) the larvae themselves; and (5) fungi associated with infestation of oranges. Habitat cues associated with host activity and those produced by rotten oranges or oranges colonized by fungi were highly attractive for female wasps, whereas odours associated with the activity of the adults on the surface of the fruit, and those released by the fruit after being damaged (as happens during fruit fly egg‐laying) were not used as cues by female parasitoids. Once the female had landed on the fruit, direct cues associated with larval activity became important although some indirect signals (e.g., products derived from larval activity inside the fruit) also increased host searching activity. Our findings indicate that naïve D. longicaudata uses chemical cues during host habitat searching and that these cues are produced both by the habitat and by the host larvae.
Instituto de Genética
Author affiliation: Segura, Diego Fernando. INTA. Instituto de Genética; Argentina
Author affiliation: Viscarret, Mariana Mabel. INTA. Instituto de Genética; Argentina. INTA. Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina
Author affiliation: Ovruski Alderete, Sergio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Carabajal Paladino, Leonale Suzel; Lo Nostro, Fabiana Laura; Papeschi, Alba Graciela; Cladera, Jorge Luis; Bressa, María José
Publication Date: 2017.
Language: English.
Abstract:
The study of spermatogenesis in Hymenoptera is limited to few taxa due to limitations to access the material in the right stage for analysis. The information available up to now states that the gametogenesis in males involves a modified meiosis, similar to mitosis. This is due to the haplodiploid sex determination system present in the order, where females are diploid and males are haploid. In the present work, we examined the spermatogenesis process in the parasitoid Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). We performed squash preparations from testes of individuals from the third larval instar until the adult stage. Some cytological traits, such as the presence of a monopolar spindle, were analysed in detail by means of histological semi-thin sections. The spermatogenesis in this species comprises an abortive first division with the formation of an anucleated cytoplasmic bud and a nucleated cell, and an equal second division of the nucleated cell, leading to the generation of two mature spermatozoa. The results presented herein provide more information about the process of spermatogenesis in Hymenoptera, which has not been studied in depth in parasitic species.
Inst. de Genética "Ewald A. Favret"- IGEAF
Author affiliation: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. INTA. Instituto de Genética; Argentina
Author affiliation: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Author affiliation: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética; Argentina
Author affiliation: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Mannino, María Constanza; Rivarola, Maximo Lisandro; Scannapieco, Alejandra Carla; Gonzalez, Sergio Alberto; Farber, Marisa Diana; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz
Publication Date: 2016.
Language: English.
Abstract:
Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid species, a deeper analysis on D. longicaudata’s genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions. Results: We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized.
Inst. de Genética "Ewald A. Favret"- IGEAF
Author affiliation: Mannino, María Constanza. INTA. Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Rivarola, Maximo Lisandro. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Scannapieco, Alejandra Carla. INTA. Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Gonzalez, Sergio Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Farber, Marisa Diana. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina
Author affiliation: Lanzavecchia, Silvia Beatriz. INTA. Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria