Authors: McMurray, John J. V.; Anand, Inder S.; Diaz, Rafael; Maggioni, Aldo P.; O'Connor, Christopher; Pfeffer, Marc A.; Solomon, Scott D.; Tendera, Micha; van Veldhuisen, Dirk J.; Moetaz, Albizem; Cheng, Sunfa; Scarlata, Debra; Swedberg, Karl; Young, James B.; Toblli, Jorge Eduardo; RED-HF Committees Investigators
Publication Date: 2013.
Language: English.
Abstract:
AIMS: This report describes the baseline characteristics of patients in the Reduction of Events with Darbepoetin alfa in Heart Failure trial (RED-HF) which is testing the hypothesis that anaemia correction with darbepoetin alfa will reduce the composite endpoint of death from any cause or hospital admission for worsening heart failure, and improve other outcomes. METHODS AND RESULTS: Key demographic, clinical, and laboratory findings, along with baseline treatment, are reported and compared with those of patients in other recent clinical trials in heart failure. Compared with other recent trials, RED-HF enrolled more elderly [mean age 70 (SD 11.4) years], female (41%), and black (9%) patients. RED-HF patients more often had diabetes (46%) and renal impairment (72% had an estimated glomerular filtration rate < 60 mL/min/1.73 m2). Patients in RED-HF had heart failure of longer duration [5.3 (5.4) years], worse NYHA class (35% II, 63% III, and 2% IV), and more signs of congestion. Mean EF was 30% (6.8%). RED-HF patients were well treated at randomization, and pharmacological therapy at baseline was broadly similar to that of other recent trials, taking account of study-specific inclusion/exclusion criteria. Median (interquartile range) haemoglobin at baseline was 112 (106-117) g/L. CONCLUSION: The anaemic patients enrolled in RED-HF were older, moderately to markedly symptomatic, and had extensive co-morbidity.
Author affiliation: McMurray, John J. V.. University of Glasgow; Reino Unido
Author affiliation: Anand, Inder S.. University of Minnesota; Estados Unidos
Author affiliation: Diaz, Rafael. Estudios Clínicos Latinoamérica; Argentina
Author affiliation: Maggioni, Aldo P.. Associazione Nazionale Medici Cardiologi Ospedalieri; Italia
Author affiliation: O'Connor, Christopher. University of Duke; Estados Unidos
Author affiliation: Pfeffer, Marc A.. Brigham and Women’s Hospita; Estados Unidos
Author affiliation: Solomon, Scott D.. Brigham and Women’s Hospital; Estados Unidos
Author affiliation: Tendera, Micha. Medical University of Silesia; Polonia
Author affiliation: van Veldhuisen, Dirk J.. University of Groningen; Países Bajos
Author affiliation: Moetaz, Albizem. Amgen Inc.; Estados Unidos
Author affiliation: Cheng, Sunfa. Amgen Inc.; Estados Unidos
Author affiliation: Scarlata, Debra. Amgen Inc.; Estados Unidos
Author affiliation: Swedberg, Karl. University of Gothenburg; Suecia
Author affiliation: Young, James B.. Cleveland Clinic. Endocrinology and Metabolism Institute; Estados Unidos
Author affiliation: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: RED-HF Committees Investigators.
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Barrandeguy, Maria Edith; Espasandin, A.G.; Alvarez, Irene; Vissani, Aldana; Martinez, D.E; Chung, S.; Tsai, Y.; Lee, A.; Wang, T.
Publication Date: 2016.
Language: English.
Abstract:
Inst.de Virología
Author affiliation: Barrandeguy, Maria Edith. INTA. Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Author affiliation: Espasandin, A.G. Universidad Nacional del Noreste. Facultad de Veterinaria; Argentina
Author affiliation: Alvarez, Irene. INTA. Instituto de Virología; Argentina
Author affiliation: Vissani, Aldana. INTA. Instituto de Virología; Argentina
Author affiliation: Martinez, D.E. Universidad Nacional del Noreste. Facultad de Veterinaria; Argentina
Author affiliation: Chung, S. GeneReach USA. Department of Research and Development; Estados Unidos
Author affiliation: Tsai, Y. GeneReach USA. Department of Research and Development; Estados Unidos
Author affiliation: Lee, A. GeneReach USA. Department of Research and Development; Estados Unidos
Author affiliation: Wang, T. GeneReach USA. Department of Research and Development; Estados Unidos
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Costa, Sofia; Sastre, Patricia; Pérez, Teresa; Tapia, Istar; Barrandeguy, Maria Eugenia; Sánchez-Vizcaíno, José M.; Sánchez-Matamoros, Almudena; Wigdorovitz, Andrés; Sanz, Antonio; Rueda, Paloma
Publication Date: 2016.
Language: English.
Abstract:
African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
Author affiliation: Costa, Sofia. Inmunología y Genética Aplicada; España
Author affiliation: Sastre, Patricia. Inmunología y Genética Aplicada; España
Author affiliation: Pérez, Teresa. Inmunología y Genética Aplicada; España
Author affiliation: Tapia, Istar. Inmunología y Genética Aplicada; España
Author affiliation: Barrandeguy, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Universidad del Salvador; Argentina
Author affiliation: Sánchez-Vizcaíno, José M.. VISAVET Health Surveillance Centre; España. Animal Health Department; España
Author affiliation: Sánchez-Matamoros, Almudena. VISAVET Health Surveillance Centre; España. Animal Health Department; España
Author affiliation: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Author affiliation: Sanz, Antonio. Inmunología y Genética Aplicada; España
Author affiliation: Rueda, Paloma. Inmunología y Genética Aplicada; España
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Costa, Sofia; Sastre, Patricia; Pérez, Teresa; Tapia, Istar; Barrandeguy, Maria Edith; Sanchez-Vizcaino, José Manuel; Sanchez-Matamoros, Almudena; Wigdorovitz, Andres; Sanz, Antonio; Rueda, Paloma
Publication Date: 2016.
Language: English.
Abstract:
African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
Inst.de Virología
Author affiliation: Costa, Sofia. Inmunología y Genética Aplicada S.A.; España
Author affiliation: Sastre, Patricia. Inmunología y Genética Aplicada S.A.; España
Author affiliation: Pérez, Teresa. Inmunología y Genética Aplicada S.A.; España
Author affiliation: Tapia, Istar. Inmunología y Genética Aplicada S.A.; España
Author affiliation: Barrandeguy, Maria Edith. INTA. Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Author affiliation: Sanchez-Vizcaino, José Manuel. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Author affiliation: Sanchez-Matamoros, Almudena. Universidad Complutense de Madrid. Facultad de Veterinaria. Departamento de Sanidad Animal; España. VISAVET Health Surveillance Centre; España
Author affiliation: Wigdorovitz, Andres. INTA. Instituto de Virología; Argentina
Author affiliation: Rueda, Paloma. Inmunología y Genética Aplicada S.A.; España
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Vissani, Aldana; Reynal O'Connor, J.; Olguin Perglione, Cecilia; Traverso, S.; Gutierrez, G.; Alvarez, Irene; Barrandeguy, Maria Edith
Publication Date: 2016.
Language: English.
Abstract:
Inst.de Virología
Author affiliation: Vissani, Aldana. INTA. Instituto de Virología; Argentina
Author affiliation: Reynal O'Connor, J. Laboratorio Equino S.R.L.; Argentina
Author affiliation: Olguin Perglione, Cecilia. INTA. Instituto de Virología; Argentina
Author affiliation: Traverso, S. Laboratorio Equino S.R.L.; Argentina
Author affiliation: Gutierrez, G. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Alvarez, Irene. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Barrandeguy, Maria Edith. INTA. Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Espasandin, A.G.; Vissani, Aldana; Martinez, D.E.; Lottero, E.; Carballo, L.P.; Storani, C.A.; Jacobo, R.A.; Cipolini, M.F.
Publication Date: 2016.
Language: English.
Abstract:
The goal of the present work was to determinate the prevalence of EIA infection among work horses in three cattle farms situated in the Northwest of Corrientes. A sample of whole blood was obtained from all the horses (n¼212), and the AGID test was carried out. One hundred and nineteen horses were found infected (AGID positive)
Inst.de Virología
Author affiliation: Espasandin, A.G. Universidad Nacional del Nordeste. Catedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Vissani, Aldana. INTA. Instituto de Virología; Argentina
Author affiliation: Martinez, D.E. Universidad Nacional del Nordeste. Catedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Lottero, E. Universidad Nacional del Nordeste. Catedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Carballo, L.P. Universidad Nacional del Nordeste. Catedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Jacobo, R.A. Universidad Nacional del Nordeste. Cátedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Cipolini, M.F. Universidad Nacional del Nordeste. Catedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Storani, C.A. Universidad Nacional del Nordeste. Cátedra de Enfermedades Infecciosas de la Facultad de Ciencias Veterinarias; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Silva e Souza, Geraldo da; Gonçalves Gomes, Eliane; Santos, Sandra Aparecida; Rezende, Adalgiza Souza Carneiro de; Freitas Andrade, Débora Roque de; Nogueira, Márcia Furlan; Trigo, Pablo Ignacio; Abreu, Urbano Gomes Pinto de
Publication Date: 2018.
Language: English.
Abstract:
This study aimed to assess the physical performance of Pantaneiro horses with and without equine infectious anemia (EIA) under functional conditions of cattle management. The horses were subjected to a performance test and split into two groups according to a completely randomized design: animals were chosen from populations testing positive and negative for EIA. Performance was measured as a function of a data envelopment analysis (DEA) model considering four outputs and one unitary input. The output measures were the distance achieved in the performance test, hematocrit as a weighted average over the test duration, respiratory rate as weighted average over the test duration, and the level of lactic acid at the test termination. Weights for the hematocrit and the respiratory rate output variables were determined by means of factor analysis. The performance score was a weighted average of the output variables with the weights defined by the averages of the optimum individual multipliers in the DEA analysis. Contextual variables of interest were age, horse weight, room temperature, and corporal temperature. Only groups and room temperature were statistically significant effects, as indicated by a bootstrap analysis. The performance of group positive for EIA is significantly lower than that of the group negative for EIA and room temperature has a negative effect.
Author affiliation: Silva e Souza, Geraldo da. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Author affiliation: Gonçalves Gomes, Eliane. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Author affiliation: Santos, Sandra Aparecida. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Author affiliation: Rezende, Adalgiza Souza Carneiro de. Universidade Federal de Minas Gerais; Brasil
Author affiliation: Freitas Andrade, Débora Roque de. Universidade Federal de Minas Gerais; Brasil
Author affiliation: Nogueira, Márcia Furlan. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Author affiliation: Trigo, Pablo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Author affiliation: Abreu, Urbano Gomes Pinto de. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: García, Enrique N.; Aguirre, María V.; Gimeno, Eduardo Juan; Rios, Elvio E.; Acosta, Ofelia Cristina; Cholich, Luciana Andrea
Publication Date: 2015.
Language: English.
Abstract:
Ipomoea carnea subsp. fistulosa (Convolvulaceae) causes poisoning of goats, sheep and cattle in many tropical and subtropical countries. The pathophysiology of this poisoning mainly involves an abnormal glycoprotein metabolism. The aim of this study was to describe the potential toxicity of I. carnea in a guinea pig model through its effect on hematopoiesis in a time course study of 40 days. Experimental poisoning was achieved by feeding animals with "small balls" prepared with milled leaves of I. carnea mixed with commercial crushed pellets for rodents. Hematologic and biochemical parameters, bone marrow and spleencellularities, histopathologic evaluations and lectin-histochemistrywere performed during the scheduled time of the study. The treatment with "small balls" caused significant changes in the weight of spleen, a notable decrease in peripheral red blood cells, and concomitantwith morphological and histopathologicalalterationsin hematopoietic tissues. Overall, the present study suggested that 20 days ofthis treatmentcouldbe enough to develop bone marrow hypoplasia and vacuolation of white cells of spleen, blood and lymph nodes with a transient erythropoietic contribution of the splenic niche. Moreover, this work provides a cheap and simple method for detecting preclinical cases of intoxication by I. carnea in livestock.
Author affiliation: García, Enrique N.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Aguirre, María V.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina
Author affiliation: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Rios, Elvio E.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Cook, R.F.; Barrandeguy, Maria Edith; Lee, Pei-Yu Alison; Tsai, Chuan-Fu; Shen, Yu-Han; Tsai, Yun-Long; Chang, Hsiao-Fen G.; Wang, Hwa-Tang Thomas; Balasuriya, Udeni B.R.
Publication Date: 2018.
Language: English.
Abstract:
Background: Control of equine infectious anaemia (EIA) currently depends on serological diagnosis of infected equids. However, recently infected equids may not produce detectable anti-EIAV antibodies up to 157 days post infection and so present a high transmission risk. Therefore, direct nucleic acid detection methods are urgently needed to improve EIAV surveillance and management programs in counties where the disease is endemic. Objectives: To evaluate a field-deployable, reverse transcription-insulated isothermal PCR (RT-iiPCR) assay targeting the conserved 50 untranslated region (50 UTR)/exon 1 of the tat gene of EIAV. Study design: The analytical and clinical performance of the newly developed EIAV RT-iiPCR was evaluated by comparison with a EIAV real-time RTPCR (RT-qPCR) along with the AGID test. Methods: Analytical sensitivity was determined using in vitro transcribed RNA containing the target area of the 50 UTR/tat gene and samples from two EIAV-positive horses. Specificity was verified using nine common equine viruses. Clinical performance was evaluated by comparison with EIAV RT-qPCR and AGID using samples derived from 196 inapparent EIAV carrier horses. Results: EIAV RT-iiPCR did not react with other commonly encountered equine viruses and had equivalent sensitivity (95% detection limit of eight genome equivalents), with a concordance of 95.41% to conventional EIAV RT-qPCR. However, the RT-qPCR and RT-iiPCR had sensitivities of 43.75 and 50.00%, respectively, when compared to the AGID test. Main limitations: Low viral loads commonly encountered in inapparent EIAV carriers may limit the diagnostic sensitivity of RT-PCR-based tests. Conclusions: Although EIAV RT-iiPCR is not sufficiently sensitive to replace the current AGID test, it can augment control efforts by identifying recently exposed or “serologically silent” equids, particularly as the latter often represent a significant transmission risk because of high viral loads. Furthermore, the relatively low cost and field-deployable design enable utilisation of EIAV RT-iiPCR even in remote regions.
Instituto de Virología
Author affiliation: Cook, R.F. Louisiana State University. School of Veterinary Medicine. Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences; Estados Unidos
Author affiliation: Barrandeguy, Maria Edith. INTA. Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Author affiliation: Lee, Pei-Yu Alison. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Tsai, Chuan-Fu. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Shen, Yu-Han. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Tsai, Yun-Long. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Chang, Hsiao-Fen G. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Wang, Hwa-Tang Thomas. GeneReach USA, Lexington; Estados Unidos
Author affiliation: Balasuriya, Udeni B.R. Louisiana State University. School of Veterinary Medicine. Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences; Estados Unidos
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria
Authors: Alvarez, Irene; Cipolini, Fabiana; Wigdorovitz, Andres; Trono, Karina Gabriela; Barrandeguy, Maria Edith
Publication Date: 2015.
Language: English.
Abstract:
The most used and reliable indicator of equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for the detection of EIAV antibodies as well as the potential advantages of their use as an EIAV infection screening tool were evaluated in 302 horse serum samples. Both ELISA assays showed 100% diagnostic sensitivity, and 92.3–94.3% diagnostic specificity. Discordant results were analyzed by immunoblot. The results showed that both ELISA tests are very efficient at detecting EIAV infected animals, allowing to identify a higher number of positive horse cases. Thus, ELISA assays can be useful tools in EIA control and eradication.
El mejor indicador de la infección por el virus de la anemia infecciosa equina (Equine infectious anemia virus, EIAV) es la detección de anticuerpos específicos en el suero del caballo. En el presente trabajo se evaluó la capacidad de detección de anticuerpos contra EIAV de dos equipos de ELISA comerciales utilizando 302 muestras de suero equino, así como las ventajas potenciales de su uso como herramientas de screening. Ambos ensayos de ELISA presentaron 100 % de sensibilidad diagnóstica y una especificidad diagnóstica del orden de 92,3 a 94,3 %. Las muestras discordantes fueron analizadas por inmunoblot. Los resultados mostraron que las dos pruebas ELISA son muy eficientes para detectar animales infectados por EIAV, al permitir identificar un mayor número de animales positivos que la prueba de inmunodifusión en gel de agar, oficialmente aprobada en la República Argentina para la certificación de los animales. Las pruebas de ELISA constituyen herramientas muy útiles en los programas de control y de erradicación de la infección por EIAV.
Instituto de Biotecnología
Author affiliation: Alvarez, Irene. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Cipolini, Fabiana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Author affiliation: Wigdorovitz, Andrés. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Trono, Karina Gabriela. INTA. Instituto de Virología; Argentina
Author affiliation: Barrandeguy, Maria Edith. INTA. Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria