Publication Date: 2010.
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy–Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations fromtwo African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data.
Author affiliation: Lanzavecchia, Silvia Beatriz. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Author affiliation: Remis, Maria Isabel. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Genética de la Estructura Poblacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Cladera, Jorge Luis. INTA. Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Author affiliation: Zandomeni, Ruben. INTA. Instituto de Microbiología y Zoología Agrícola. Laboratorio de Alta Complejidad; Argentina
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria