Authors: González, Noelia; Gil, María Lydia; Rios, Glenda Laura; Canizo, Jésica Romina; De Blas, José Ignacio; Alberio, Ricardo
Publication Date: 2014.
Language: English.
Abstract:
The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.
EEA Balcarce
Author affiliation: González, Noelia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Author affiliation: Gil, Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España
Author affiliation: Rios, Glenda Laura. INTA. Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Author affiliation: Canizo, Jésica Romina. INTA. Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Author affiliation: De Blas, José Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Infectious Diseases Unit; España
Author affiliation: Alberio, Ricardo. INTA. Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Keywords: Óvulo; Perro; Experimentación in Vitro; Ova; Dogs; In Vitro Experimentation; Ovocitos; Atmósfera Gaseosa.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria