Publication Date: 2015.
The objectives of this study were to compare the effects of two intravaginal devices (ID) containing the same dose (0.5 g) of progesterone (P4) on subsequent ovarian response, embryo production and circulating P4 concentration profile in llamas (Lama glama) treated with equine chorionic gonadotropin (eCG) for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group) to one of the following groups and treated (Day 0) with an ID containing 0.5 g of vegetal P4 to synchronize the emergence of a new follicular wave: i) DIB 0.5® and ii) Cronipres M15®. On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9) and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P4 concentrations. The results indicated that DIB device maintained greater plasma P4 levels as compared to Cronipres until Day 2. However, the mean (± SD) number of corpora lutea and recovered embryos was not affected (p < 0.05) by the type of ID (5.3 ± 2.6 vs 4.2 ± 2.2 and 3.5 ± 2.7 vs 2.6 ± 3.0 for DIB and Cronipres, respectively). In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas.
Author affiliation: Aller Atucha, Juan Florencio. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Author affiliation: Abalos, Marcos. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina
Author affiliation: Acuña, Francisco Antonio. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina
Author affiliation: Virgili, Rosana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; Argentina
Author affiliation: Requena, Francisco. Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario Rabanales; España
Author affiliation: Cancino, Andrea Karina. INTA. Estación Experimental Agropecuaria Bariloche; Argentina
Keywords: Embriones Animales; Animal Embryos; Superovulation; Hormonal Regulation; Superovulación; Progesterona; Llama; Lama Glama; PMSG; Regulación Hormonal; Gonadotropina Coriónica Equina; Dispositivos Intravaginales; Tratamiento Hormonal; eCG; Intravaginal Devices; Equine Chorionic Gonadotropin; Hormonal Treatment.
Repository: INTA Digital (INTA). Instituto Nacional de Tecnología Agropecuaria