Authors: de Oliveira Silva, Franklin Magnum; Lichtenstein, Gabriel; Alseekh, Saleh; Rosado Souza, Laise; Conte, Mariana; Suguiyama, Vanessa Fuentes; Lira, Bruno Silvestre; Fanourakis, Dimitrios; Usadel, Björn; Bhering, Leonardo Lopes; DaMatta, Fábio M.; Sulpice, Ronan; Araújo, Wagner L.; Rossi, Magdalena; de Setta, Nathalia; Fernie, Alisdair R.; Carrari, Fernando Oscar; Nunes Nesi, Adriano
Publication Date: 2018.
Language: English.
Abstract:
To identify genomic regions involved in the regulation of fundamental physiological processes such as photosynthesis and respiration, a population of Solanum pennellii introgression lines was analyzed. We determined phenotypes for physiological, metabolic, and growth related traits, including gas exchange and chlorophyll fluorescence parameters. Data analysis allowed the identification of 208 physiological and metabolic quantitative trait loci with 33 of these being associated to smaller intervals of the genomic regions, termed BINs. Eight BINs were identified that were associated with higher assimilation rates than the recurrent parent M82. Two and 10 genomic regions were related to shoot and root dry matter accumulation, respectively. Nine genomic regions were associated with starch levels, whereas 12 BINs were associated with the levels of other metabolites. Additionally, a comprehensive and detailed annotation of the genomic regions spanning these quantitative trait loci allowed us to identify 87 candidate genes that putatively control the investigated traits. We confirmed 8 of these at the level of variance in gene expression. Taken together, our results allowed the identification of candidate genes that most likely regulate photosynthesis, primary metabolism, and plant growth and as such provide new avenues for crop improvement.
Author affiliation: de Oliveira Silva, Franklin Magnum. Universidade Federal de Viçosa; Brasil
Author affiliation: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Author affiliation: Alseekh, Saleh. Max Planck Institute of Molecular Plant Physiology; Alemania
Author affiliation: Rosado Souza, Laise. Max Planck Institute of Molecular Plant Physiology; Alemania
Author affiliation: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Author affiliation: Suguiyama, Vanessa Fuentes. Universidade Federal do ABC; Brasil
Author affiliation: Lira, Bruno Silvestre. Universidade de Sao Paulo; Brasil
Author affiliation: Fanourakis, Dimitrios. Vegetable Crops and Plant Protection; Grecia
Author affiliation: Usadel, Björn. RWTH Aachen University; Alemania. Forschungszentrum Jülich; Alemania
Author affiliation: Bhering, Leonardo Lopes. Universidade Federal de Viçosa; Brasil
Author affiliation: DaMatta, Fábio M.. Universidade Federal de Viçosa; Brasil
Author affiliation: Sulpice, Ronan. National University of Ireland Galway; Irlanda
Author affiliation: Araújo, Wagner L.. Universidade Federal de Viçosa; Brasil
Author affiliation: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
Author affiliation: de Setta, Nathalia. Universidade Federal do ABC; Brasil
Author affiliation: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania
Author affiliation: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Author affiliation: Nunes Nesi, Adriano. Universidade Federal de Viçosa; Brasil
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas