Genetic diversity of Vibrio cholerae O1 in Argentina and emergence of a new variant

Authors
Pichel, Mariana; Rivas, Marta; Chinen, Isabel; Martín, Fernando A.; Ibarra, Cristina; Binsztein, Norma
Publication Year
2003
Language
English
Format
article
Status
Published version
Description
The genetic diversity of Vibrio cholerae O1 strains from Argentina was estimated by random amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). Twenty-nine isolates carrying the virulence genes ctxA, zot, ace, and tcpA appeared to represent a single clone by both typing methods; while 11 strains lacking these virulence genes exhibited several heterogeneous RAPD and PFGE patterns. Among the last group, a set of isolates from the province Tucuma´n showed a single RAPD pattern and four closely related PFGE profiles. These strains, isolated from patients with diarrhea, did not produce the major V. cholerae O1 virulence determinants, yet cell supernatants of these isolates caused a heat-labile cytotoxic effect on Vero and Y-1 cells and elicited significant variations on the water flux and short-circuit current in human small intestine mounted in an Ussing chamber. All these effects were completely abolished by incubation with a specific antiserum against El Tor hemolysin, suggesting that this virulence factor was responsible for the toxic activity on both the epithelial cells and the small intestine specimens and may hence be involved in the development of diarrhea. We propose “Tucuma´n variant” as the designation for this new cluster of cholera toxin-negative V. cholerae O1 strains.
Fil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
Fil: Chinen, Isabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
Fil: Martín, Fernando. Universidad de Buenos Aires. Departamento de Fisiología; Argentina.
Fil: Ibarra, Cristina. Universidad de Buenos Aires. Departamento de Fisiología; Argentina.
Fil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
Source
Journal of Clinical Microbiology, 2003, 41(1), 124–134.
Subject
Vibrio cholerae
Cólera
Diarrea
Variación Genética
Humanos
Argentina
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repository
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institution
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identifier
oai:repositorio.anlis.gov.ar:123456789/281