Molecular epidemiology of vancomycin-resistant Enterococcus faecium in Argentina

Authors
Corso, Alejandra; Gagetti, Paula; Rodríguez, Marisa M.; Melano, Roberto; Ceriana, Paola G.; Faccone, Diego; Galas, Marcelo F.; The VRE Argentinean Collaborative Group
Publication Year
2007
Language
English
Format
article
Status
Published version
Description
OBJECTIVE To characterize the mechanism of glycopeptide resistance and to determine the genetic relatedness among strains by pulsed-field gel electrophoresis (PFGE) in vancomycin-resistant Enterococcus faecium from Argentina. MATERIALS AND METHODS A total of 189 vancomycin-resistant single-patient isolates of Enterococcus faecium recovered between January 1997 and December 2000 from 30 hospitals in Argentina were studied. Minimum inhibitory concentrations were determined by the agar dilution method and van genes were detected by PCR. PFGE was used for molecular typing. RESULTS All isolates except three (vanB) were of genotype vanA. For 189 vancomycin-resistant Enterococcus faecium, SmaI-PFGE indicated 35 clonal types. Most of the isolates (56%) belonged to the same clonal type 1, which was present in 19 hospitals and dominant in 17. CONCLUSIONS The emergence of vancomycin-resistant Enterococcus faecium in Argentina seems to be related to the intra- and inter-hospital dissemination of an epidemic clone carrying the vanA element.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Gagetti, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Rodríguez, Marisa M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Melano, Roberto. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Ceriana, Paola G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Galas, Marcelo F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: The VRE Argentinean Collaborative Group; Argentina.
Source
International Journal of Infectious Diseases, 2007, 11(1), 69-75.
Subject
Enterococcus faecium
Epidemiología Molecular
Resistencia a la Vancomicina
Argentina
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repository
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institution
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identifier
oai:repositorio.anlis.gov.ar:123456789/424