Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity

Authors
Capitaine Funes, Juan; Massa, Estefania; Cipulli, Germán; Benitez Gil, Alfonso; Capitaine Funes, Carlos; Tozzini, Roberto; Ghersevich, Sergio; Ceballos, María Paula
Publication Year
2014
Language
English
Format
article
Status
Versión aceptada para publicación
Description
It is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treatment patients”; n = 47) and after (“post-treatment patients”; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0-4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analysis. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. Results showed a significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001). The % head DNA in post-treatment samples from patients with high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patient with low to medium level of DNA damage before therapy (49.2 ± 4.4%). Results support the usefulness of the comet assay as a sensitive technique to evaluate the basal DNA status and the damage caused by cancer treatments. The assay could contribute to the treatment decisions, taking into account the patients’ basal DNA damage before therapy.
Fil: Capitaine Funes, Juan. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Ceballos, María Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (UNR-CONICET); Argentina
Fil: Capitaine Funes, Juan. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina
Fil: Massa, Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Cipulli, Germán. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina
Fil: Benitez Gil, Alfonso. Provincia de Santa Fe. Hospital Provincial. Servicio de Mastología; Argentina
Fil: Capitaine Funes, Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Capitaine Funes, Carlos. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina
Fil: Tozzini, Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Tozzini, Roberto. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina
Fil: Ghersevich, Sergio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Subject
Ensayo cometa
Daño genotóxico
Cáncer mamas
Factor pronóstico
Peripheral blood lymphocytes
Comet assay
Genotoxic damage
Access level
Open access
License
Repository
RepHipUNR (UNR)
Institution
Universidad Nacional de Rosario
OAI Identifier
oai:rephip.unr.edu.ar:2133/9378