Comparative histopathological and immunological study of two field strains of chicken anemia virus

Authors
Rimondi, Agustina; Pinto, Silvina; Olivera, Valeria Soledad; Dibarbora, Marina; Perez Filgueira, Daniel Mariano; Craig, María Isabel; Pereda, Ariel Julian
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.
Instituto de Virología
Fil: Rimondi, Agustina. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología Aviar; Argentina
Fil: Olivera, Valeria Soledad. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Dibarbora, Marina. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Craig, María Isabel. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Pereda, Ariel Julian. INTA. Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Source
BMC veterinary research 45 : 102. (2014)
Subject
Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3582