Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure

Authors
Perotti, Valeria Elisa; Moreno, Alejandra Soledad; Tripodi, Karina Eva Josefina; Meier, Guillermo Enrique; Bello, Fernando; Cocco, Mariángeles; Vazquez, Daniel Eduardo; Anderson, Catalina Margarita; Podesta, Florencio Esteban
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography‐mass spectrometry and liquid chromatography‐mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost‐exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold‐sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
EEA Concordia
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo Enrique. INTA. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. INTA. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Cocco, Mariángeles. INTA. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. INTA. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Anderson, Catalina Margarita. INTA. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Source
Physiologia Plantarum 153 (3) : 337-354 (March 2015)
Subject
Naranja Dulce
Variedades
Citrus
Helada
Estrés de Frío
Metabolismo
Sweet Oranges
Varieties
Frost
Cold Stress
Metabolism
Variedad Valencia
Proteomics
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4653