Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)

Authors
Moschen, Sebastian Nicolas; Di Rienzo, Julio A.; Higgins, Janet; Tohge, Takayuki; Watanabe, Mutsumi; Gonzalez, Sergio Alberto; Rivarola, Maximo Lisandro; García-García, Francisco; Dopazo, Joaquín; Hopp, Horacio Esteban; Hoefgen, Rainer; Fernie, Alisdair R.; Paniego, Norma Beatriz; Fernandez, Paula Del Carmen; Heinz, Ruth Amelia
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach. Our results showed a delay in plant senescence with an increase in the expression level of photosynthesis related genes as well as higher levels of sugars, osmoprotectant amino acids and ionic nutrients under drought conditions. In addition, we identified transcription factors that were upregulated during drought conditions and that may act as hubs in the transcriptional network. Many of these transcription factors belong to families implicated in the drought response in model species. The integration of transcriptomic and metabolomic data in this study, together with physiological measurements, has improved our understanding of the biological responses during droughts and contributes to elucidate the molecular mechanisms involved under this environmental condition. These findings will provide useful biotechnological tools to improve stress tolerance while maintaining crop yield under restricted water availability.
Instituto de Biotecnología
Fil: Moschen, Sebastian Nicolas. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Rienzo, Julio A.Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Higgins, Janet. Norwich Research Park. Earlham Institute; Reino Unido
Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Watanabe, Mutsumi Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Gonzalez, Sergio Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Maximo Lisandro. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España
Fil: Dopazo, Joaquín. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España
Fil: Hopp, Horacio Esteban. INTA. Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Hoefgen, Rainer Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Paniego, Norma Beatriz. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula Del Carmen. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Heinz, Ruth Amelia. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Source
Plant molecular biology 94 (4–5) : 549–564. (July 2017)
Subject
Helianthus Annuus
Metabolismo
Genética
Sequía
Estrés de Sequia
Metabolism
Genetics
Drought
Drought Stress
Girasol
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1960