Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation

Authors
Beati, Lorenza; Nava, Santiago; Burkman, Erica J.; Barros Battesti, Darci M.; Labruna, Marcelo B.; Guglielmone, Alberto; Cáceres, Abraham G.; Guzman Cornejo, Carmen M.; Léon, Renato; Durden, Lance A.; Faccini, João L.H.
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Background: Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Results: Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Conclusions: Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.
EEA Rafaela
Fil: Beati, Lorenza. Georgia Southern University. Institute for Coastal Plain Science. United States National Tick Collection; Estados Unidos
Fil: Nava, Santiago. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Burkman, Erica J. Georgia Southern University. Institute for Coastal Plain Science. United States National Tick Collection; Estados Unidos. University of Georgia. College of Veterinary Medicine. Department of Infectious Diseases; Estados Unidos
Fil: Barros Battesti, Darci M. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan. Laboratório de Parasitologia; Brasil
Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Guglielmone, Alberto Alejandro. INTA. Estación Experimental Regional Agropecuaria Rafaela; Argentina
Fil: Cáceres, Abraham G. Universidad Nacional Mayor de San Marco. Facultad de Medicina. Departamento Académico de Microbiologia Médica; Perú. Instituto Nacional de Salud. Laboratorio de Entomología; Perú
Fil: Guzman Cornejo, Carmen. Universidad Nacional Autónoma de México. Facultad de Ciencias. Departamento de Biología Comparada. Laboratorio de Acarología; México
Fil: Léon, Renato. Universidad San Francisco de Quito. Colegio de Ciencias Biológicas y Ambientales. Laboratorio de Entomología Médica y Medicina Tropical (LEMMT); Ecuador
Fil: Durden, Lance A. Georgia Southern University. Biology Department; Estados Unidos
Fil: Faccini, João L.H. Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Parasitologia Animal; Brasil
Source
BMC Evolutionary Biology 13 : 267 (2013)
Subject
Amblyomma cajennense
Genética
Distribución Geográfica
Ecosistema
Genetics
Geographical Distribution
Ecosystems
Garrapatas
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2995