Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine...

Authors
Del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Esusy, María Soledad; Federico, Carlos Rodolfo; Zabal, Osvaldo Alfredo; Valera, Alejandro Rafael; Calamante, Gabriela
Publication Year
2017
Language
English
Format
article
Status
Versión aceptada para publicación
Description
In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.
Inst. de Biotecnología
Fil: Del Medico Zajac, Maria Paula. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zanetti, Flavia Adriana. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esusy, María Soledad. INTA. Instituto de Biotecnología; Argentina
Fil: Federico, Carlos Rodolfo. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabal, Osvaldo Alfredo. INTA. Instituto de Virología; Argentina
Fil: Valera, Alejandro Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Virología; Argentina
Fil: Calamante, Gabriela. INTA. Instituto de Biotecnología; Argentina
Source
Viral immunology 30 (1) : 70-76. (2017)
Subject
Enfermedades de los Animales
Respuesta inmunológica
Ratón
Herpes Virus Bovino
Glicoproteínas
Immune response
Animal Diseases
Mice
Bovine herpesvirus
Glycoproteins
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/960