A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Authors
Hecker, Yanina; Cóceres, Verónica Mabel; Wilkowsky, Silvina Elizabeth; Jaramillo Ortiz, Jose Manuel; Morrell, Eleonora Lidia; Verna, Andrea Elizabeth; Ganuza, Agustina; Cano, Dora Beatriz; Lischinsky, Lilian Haydee; Ángel, Sergio Oscar; Zamorano, Patricia Ines; Odeon, Anselmo Carlos; Leunda, Maria Rosa; Campero, Carlos Manuel; Morein, Bror; Moore, Prando Dadin
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified nickel resin. All groups were intravenously challenged with the NC-1 strain of N. caninum at Day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P < 0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all foetuses of groups A, B and C by Western blot, immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the foetal infection in pregnant cattle challenged at Day 70 of gestation.
EEA Balcarce
Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Instituto de Biotecnología; Argentina
Fil: Jaramillo Ortiz, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Instituto de Biotecnología; Argentina
Fil: Morrell, Eleonora Lidia. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina
Fil: Cano, Dora Beatriz. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Lischinsky, Lilian Haydee. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina
Fil: Zamorano, Patricia Ines. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeon, Anselmo Carlos. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Leunda, Maria Rosa. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Campero, Carlos Manuel. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; Suecia
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Source
Veterinary Immunology and Immunopathology 162 (3–4) : 142-153 (December 2014)
Subject
Ganado Bovino
Gestación
Vacuna
Neospora caninum
Anticuerpos
Inmunidad Maternal
Cattle
Pregnancy
Vaccines
Antibodies
Maternal Immunity
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4360