Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers

Authors
González Altamiranda, Erika Analía; Kaiser, German Gustavo; Mucci, Nicolas Crescencio; Verna, Andrea Elizabeth; Campero, Carlos Manuel; Odeon, Anselmo Carlos
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization procedures. Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to 36 months of age were spayed and their ovaries used for viral isolation, microscopic examination, and in vitro fertilization procedures. Bovine Viral Diarrhea Virus was detected from the follicular fluid and sera of all PI heifers. Microscopic examination of the ovaries from PI heifers showed a significant drop in the number of follicles cortical regions, compared with controls. A comparative analysis of the stages of follicular development showed a significant decrease in the number of primordial and tertiary follicles in the cortical regions of ovaries from PI heifers. Viral antigen was detected by immunohistochemistry, and was widely distributed throughout the ovarian tissues. There were differences in the rate of cleavage and embryo development between oocytes obtained from the ovaries of control animals and PI heifers. Furthermore, two developed embryos obtained from oocytes from one of the PI heifers were positive to BVDV, as well as two media from in vitro fertilization (IVF) procedures. The results of this study demonstrate that BVDV PI heifers exhibit alterations in follicular population through of the early interaction between the virus and germ cell line affecting directly the mechanisms involved in the ontogenesis of the ovary.
EEA Balcarce
Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Kaiser, German Gustavo. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Mucci, Nicolas Crescencio. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Verna, Andrea Elizabeth. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Campero, Carlos Manuel. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Odeon, Anselmo Carlos. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Source
Veterinary Microbiology 165 (3–4) : 326-332 (August 2013)
Subject
Novilla
Enfermedades de los Animales
Pestivirus de la Diarrea Bovina
Diarrea Viral Bovina
Folículos Ováricos
Heifers
Animal Diseases
Bovine Diarrhoea Pestivirus
Bovine Viral Diarrhoea
Ovarian Follicles
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4329