Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury

Authors
Bustamante, Claudia Anabel; Brotman, Yavid; Monti, Laura L.; Gabilondo, Julieta; Budde, Claudio Olaf; Lara, María Valeria; Fernie, Alisdair R.; Drincovich, María Fabiana
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response.
EEA San Pedro
Fil: Bustamante, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Brotman, Yariv. Ben Gurion University of the Negev. Department of Life Sciences; Israel
Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Lara, Maria V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, Maria Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Source
Physiologia plantarum, vol. 163. 2018. p. 2-17
Subject
Durazno
Prunus persica
Almacenamiento en Frío
Fisiologia Postcosecha
Metabolismo de Lípidos
Daño por Frío
Variedades
Maduración en Postcosecha
Peaches
Cold Storage
Postharvest Physiology
Lipid Metabolism
Chilling Injury
Varieties
Postharvest Ripening
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2604