Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

Authors
Coluccio Leskow, Carla; Dominguez, Pia Guadalupe; Díaz Zirpolo, José Antonio; Obata, Toshihiro; Kamenetzky, Laura; Costa, Hernán; Taboga, Oscar Alberto; Marti, Hector Ruben; Keurentjes, Joost; Sulpice, Ronan; Ishihara, Hirofumi; Stitt, Mark; Fernie, Alisdair R.; Carrari, Fernando
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Inst. de Biotecnología
Fil: Coluccio Leskow, Carla. INTA. Instituto de Biotecnología; Argentina
Fil: Dominguez, Pia Guadalupe. INTA. Instituto de Biotecnología; Argentina
Fil: Díaz Zirpolo, José Antonio. INTA. Instituto de Biotecnología; Argentina
Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Kamenetzky, Laura. INTA. Instituto de Biotecnología; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Taboga, Oscar Alberto. INTA. Instituto de Biotecnología; Argentina
Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Holanda
Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando. INTA. Instituto de Biotecnología; Argentina
Source
Journal of experimental botany 67 (14) : 4091–4103 (July 2016)
Subject
Fitomejoramiento
Arabidopsis
Biomasa
Loci de Rasgos Cuantitativos
Plant Breeding
Biomass
Quantitative Trait Loci
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1187