Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time

Authors
Decima Oneto, Cecilia Andrea; Bossio, Adrian Ezequiel; Faccio, Paula Daniela; Beznec, Ailin; Blumwald, Eduardo; Lewi, Dalia Marcela
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Plant stress studies are increasingly being based on gene expression. The analysis of gene expression requires sensitive, precise, and replicable measurements for specific mRNA sequences. Real-time RT-PCR is nowadays the most sensitive method for the detection of low abundance mRNA. A stable reference gene is mandatory to obtain reliable quantitative real-time PCR (qPCR) analysis results. Real-time RT-PCR is referred with one or several internal control genes, which should not fluctuate during treatments. In this study, we have chosen eight genes as candidates of possible reference genes for maize (Zea mays L) during water deficit stress at flowering time: a-tubulin, 3`phosphate glyceraldehyde dehydrogenase (GAPDH), 18S ribosomal subunit, protein 13S ribosomal, actin, zein, invertase, and starch synthase IIB. The eight reference genes candidates were tested on maize plants around flowering time, under three different conditions: before water deficit (BWD), under water deficit (WD) and after water deficit (AWD). Results from the three experimental conditions indicated that protein 13S ribosomal gene was the most stable among all the reference genes tested. This result suggests that protein 13S ribosomal gene can be used as internal control (housekeeping) for qPCR analysis in maize plants under water deficit stress during flowering time.
Inst. de Genética "Ewald A. Favret"- IGEAF
Fil: Decima Oneto, Cecilia Andrea. INTA. Instituto de Genética; Argentina
Fil: Bossio, Adrian Ezequiel. INTA. Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. INTA. Instituto de Genética; Argentina
Fil: Beznec, Ailin. INTA. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blumwald, Eduardo. University of California Davies. Department Plant Sciences; Estados Unidos
Fil: Lewi, Dalia Marcela. INTA. Instituto de Genética; Argentina
Source
Maydica 62 : 6 p.
Subject
Genética
Genes
Estrés de Sequia
Floración
Genetics
Frought Stress
Flowering
Genes de Limpieza
Estres Hídrico
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/941