Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide

Authors
Villar, Margarita; Popara, Marina; Mangold, Atilio Jose; Fuente, José de la
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species.
EEA Rafaela
Fil: Villar, Margarita. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Popara, Marina. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Mangold, Atilio Jose. INTA. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Fuente, José de la. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos
Source
Journal of Proteomics 105 : 204-216 (June 2014)
Subject
Amblyomma
Vectores
Zoonosis
Huéspedes
Vectors
Zoonoses
Hosts
Garrapatas
Proteómica
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3039