High hydrostatic pressure assisted enzymatic hydrolysis of whey proteins

Ambrosi, Vanina; Polenta, Gustavo Alberto; Gonzalez, Claudia Beatriz; Ferrari, G.; Maresca, P.
Publication Year
Published version
Whey proteins, due to their high nutritional value, are generally hydrolyzed to reduce the allergenicity and used as ingredients in many special products, such as infant formulae, geriatric products, highly energetic supplements or dietetic foods or in foods produced to prevent nutrition related diseases, like food intolerances and allergies. The aim of this work was to assess the applicability of innovative technologies, such as high hydrostatic pressure (HHP) processes, to assist the enzymatic hydrolysis of target proteins, namely whey protein concentrate (WPC-80), in order to modify their antigenicity. Experiments were carried out to verify the effectiveness of HHP technology to accelerate whey protein hydrolysis reaction with selected enzymes (α-chymotrypsin, bromelain), and to affect the protein allergenic power. To this purpose, different HHP treatments were carried out at several pressure levels (100, 200, 300 and 400 MPa) and the untreated whey protein samples were used as control. A defined enzyme/substrate ratio of 1/10 w/w was used in the experiments, while the treatment time was changed from 0 to 30 min (0, 5, 15, or 30 min). The experimental data demonstrated that High Hydrostatic Pressure (HHP) induced WPC-80 unfolding at the highest value of the pressure applied (400 MPa) as indicated by the higher exposure of free sulfhydryl groups. When HHP was used in combination with enzymatic hydrolysis, the degree of hydrolysis increased not only with the pressure level applied but also with the processing time. These results suggested that, even if the exposure of hidden epitopes upon protein unfolding increased the antigenicity of whey proteins, further peptide bonds cleavage also took place after hydrolysis. This effect could modify whey proteins antigenic sequences, and thus their antigenic power.
Instituto de Tecnología de Alimentos
Fil: Ambrosi, Vanina. INTA. Instituto Tecnología de Alimentos; Argentina
Fil: Polenta, Gustavo Alberto. INTA. Instituto Tecnología de Alimentos; Argentina
Fil: Gonzalez, Claudia Beatriz . INTA. Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Ferrari, G. University of Salerno. Department of Industrial Engineering; Italia. ProdAI Scarl; Italia
Fil: Maresca, P. ProdAI Scarl; Italia
Innovative food science & emerging technologies 38, Part B : 294-301. (December 2016)
Proteínas de Suero de Leche
Procesamiento de Alimentos
Tecnología Alta Presión
Presión Hidrostática
Hidrólisis Enzimática
Whey Protein
Food Processing
High Pressure Technology
Hydrostatic Pressure
Enzymatic Hydrolysis
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Restricted access
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
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