Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage

Authors
Müller, Gabriela L.; Budde, Claudio Olaf; Lauxmann, Martín Alexander; Triassi, Agustina; Andreo, Carlos Santiago; Drincovich, María Fabiana; Lara, María Valeria
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene.
EEA San Pedro
Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Budde, Claudio Olaf. INTA. Estación Experimental Agropecuaria San Pedro; Argentina.
Fil: Lauxmann, Martin A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
Source
Functional Plant Biology 40 (5) : 449-459. (2013)
Subject
Tomate
Solanum Lycopersicum
Fisiología Postcosecha
Maduración en Postcosecha
Pared Celular
Metabolismo
Almacenamiento en Frío
Aptitud para la Conservación
Tomatoes
Postharvest Physiology
Postharvest Ripening
Cell Walls
Metabolism
Cold Storage
Keeping Quality
Metabolismo de la Pared Celular
Daño por Frío
Endotransglicosilasa/Hidrolasa
Xyloglucan β-xilosidasa
Cell Wall Metabolism
Chilling Injury
Endotransgylcosylase/Hydrolase
Xyloglucan β-xylosidase
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/3213