Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs

Authors
Pizzolitto, Romina P.; Armando, María R.; Combina, Mariana; Cavaglieri, Lilia Reneé; Dalcero, Ana María; Salvano, Mario A.
Publication Year
2012
Language
English
Format
article
Status
Versión aceptada para publicación
Description
In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host.
Fil: Pizzolitto, Romina P. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Armando, María R. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Combina, Mariana. INTA. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Salvano, Mario A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
Source
Journal of environmental science and health. Part B 47 (10) : 933-941. (2012)
Subject
Saccharomyces Cerevisiae
Aflatoxinas
Aves de Corral
Piensos
Aflatoxins
Poultry
Feeds
Probiotics
Probióticos
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1694