Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells

Authors
González Altamiranda, Erika Analía; Kaiser, German Gustavo; Rios, Glenda Laura; Leunda, Maria Rosa; Odeon, Anselmo Carlos
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.
EEA Balcarce
Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Kaiser, German Gustavo. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Rios, Glenda Laura. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Leunda, Maria Rosa. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Odeón, Anselmo Carlos. INTA. Estación Experimental Agropecuaria Balcarce; Argentina
Source
Theriogenology 86 (8) : 1999-2003 (November 2016)
Subject
Enfermedades de los Animales
Ganado Bovino
Diarrea Viral Bovina
Pestivirus de la Diarrea Bovina
Óvulo
Animal Diseases
Cattle
Bovine Viral Diarrhoea
Bovine Diarrhoea Pestivirus
Ova
Ovocitos
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4570