Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits

Authors
Pesce, Virginia Mercedes; Nally, María Cristina; Carrizo, Gabriela P.; Rojo, María Cecilia; Perez, Beatriz Alida; Toro, Maria Eugenia; Castellanos de Figueroa, Lucía Inés; Vazquez, Fabio
Publication Year
2017
Language
English
Format
article
Status
Versión aceptada para publicación
Description
Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes
Inst. de Microbiología y Zoología Agrícola IMyZA
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Carrizo, Gabriela P. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rojo, María Cecilia. INTA. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Beatriz Alida. INTA. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Source
Biological control 120 : 43-51. (May 2018)
Subject
Enfermedades de las Plantas
Colletotrichum
Aceituna
Levadura
Control Biológico
Plant Diseases
Olives
Yeasts
Biological Control
Colletotrichum Gloeosporioides
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1499