Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids

Authors
Mignaqui, Ana Clara; Ruiz, Vanesa; Perret, Sylvie; St-Laurent, Gilles; Chahal, Parminder Singh; Transfiguracion, Julia; Sammarruco, Romina Ayelén; Gnazzo, Victoria; Durocher, Yves; Wigdorovitz, Andres
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Mignaqui, Ana Clara. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ruiz, Vanesa. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Sammarruco, Romina Ayelén. INTA. Instituto de Virología; Argentina
Fil: Gnazzo, Victoria. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Wigdorovitz, Andres. INTA. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Source
PLoS ONE 8 (8) : e72800. (August 20, 2013)
Subject
Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4809