Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle

Authors
Manes, Jorgelina; Fiorentino, María Andrea; San Martino, Silvina; Ungerfeld, Rodolfo
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13.
Fil: Manes, Jorgelina. INTA. Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
Fil: Fiorentino, María Andrea. INTA. Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fil: San Martino, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Introducción a las Ciencias Agrarias. Estadística y Diseño Experimental; Argentina
Fil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
Source
Livestock science 208 : 55–59. (2018)
Subject
Flora Microbiana
Vagina
Sincronización del Celo
Ciclo Estral
Vaginitis
Oveja
Ewes
Oestrous Cycle
Oestrus Synchronization
Microbial Flora
Microbiota Vaginal
Esponjas Intravaginales
Intravaginal Sponges
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2759