Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples

Authors
Jauregui, Gloria R.; Fontana, Diego; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar
Publication Year
2019
Language
English
Format
article
Status
Published version
Description
Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
Instituto de Patobiología
Fil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; Argentina
Source
Brazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019)
Subject
Rabies Virus
Immunohistochemistry
Diagnostic Techniques
Cattle
Virus de la Rabia
Inmunohistoquímica
Técnicas de Diagnosis
Ganado Bovino
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/6311