VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display

Authors
Peralta, Andrea Veronica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
Instituto de Biotecnología
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Maria Paula. INTA. Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Source
Virus Research 175 (1) : 87-90 (July 2013)
Subject
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Ganado
Baculovirus
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Livestock
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/4355