Differential replication of Foot-and-mouth disease viruses in mice determine lethality

Authors
Cacciabue, Marco Polo; Garcia Nuñez, Maria Soledad; Delgado, Fernando Oscar; Curra, Anabella Paola; Marrero Diaz De Villegas, Rubén; Molinari, Maria Paula; Rieder, Elizabeth; Carrillo, Elisa Cristina; Gismondi, Maria Ines
Publication Year
2017
Language
English
Format
article
Status
Versión aceptada para publicación
Description
Adult C57BL/6J mice have been used to study Foot-and-mouth disease virus (FMDV) biology. In this work, two variants of an FMDV A/Arg/01 strain exhibiting differential pathogenicity in adult mice were identified and characterized: a non-lethal virus (A01NL) caused mild signs of disease, whereas a lethal virus (A01L) caused death within 24–48 h independently of the dose used. Both viruses caused a systemic infection with pathological changes in the exocrine pancreas. Virus A01L reached higher viral loads in plasma and organs of inoculated mice as well as increased replication in an ovine kidney cell line. Complete consensus sequences revealed 6 nonsynonymous changes between A01L and A10NL genomes that might be linked to replication differences, as suggested by in silico prediction studies. Our results highlight the biological significance of discrete genomic variations and reinforce the usefulness of this animal model to study viral determinants of lethality.
Inst. de Biotecnología
Fil: Cacciabue, Marco Polo. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia Nuñez, Maria Soledad. INTA. Instituto de Biotecnología; Argentina
Fil: Delgado, Fernando Oscar. INTA. Instituto de Patobiología; Argentina
Fil: Curra, Anabella Paola. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marrero Diaz De Villegas, Rubén. INTA. Instituto de Biotecnología; Argentina
Fil: Molinari, Maria Paula. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrillo, Elisa Cristina. INTA. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gismondi, Maria Ines. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Source
Virology 509 : 195-204. (September 2017)
Subject
Ratón
Fiebre Aftosa
Patogénesis
Mice
Foot and Mouth Disease
Pathogenesis
Access level
Open access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/1464